Journal
CHEMICO-BIOLOGICAL INTERACTIONS
Volume 233, Issue -, Pages 139-146Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2015.03.021
Keywords
MSC; Ca2+; iTRAQ; Proteomics; Osteogenic differentiation
Funding
- Key Clinical Specialty Discipline Construction Program of Fujian, P.R.C.
Ask authors/readers for more resources
Human mesenchymal stem cells-bone marrow (BM-hMSCs) are considered as the most suitable seed cells for bone tissue engineering. Calcium ions (Ca2+) forms an important component of a number of commercial bone substitutes and support materials. For efficient bone tissue engineering, it is crucial to explore the effect of extracellular Ca2+ on the growth and differentiation of BM-hMSCs, and to understand their molecular mechanisms. Therefore, in the present study, BM-hMSCs were cultivated in serum free growth medium or serum free growth medium with additional 4 or 6 mM Ca2+ for 3 weeks, following which, the proliferation and osteoblastic differentiation of these cells were evaluated. Differentially expressed proteins were established using iTRAQ labeling coupled with nano-LC-MS/MS. Our data revealed that Ca2+ significantly promoted the proliferation of BM-hMSCs in the early stage. Furthermore, Ca2+ showed osteoinduction properties. MAPKs signaling pathway might participate in the osteogenic differentiation of BM-hMSCs caused by Ca2+. Certain newly found proteins could be potentially important for the osteogenic differentiation of BM-hMSCs and may be associated with osteogenesis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available