Journal
METHODS AND PROTOCOLS
Volume 6, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/mps6050080
Keywords
rare cells; single-cell proteomics; microfluidics; CTC isolation; CTC collection
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Advancements in single-cell proteomics have opened new possibilities for analyzing rare cells. Efficient sample preparation is crucial for the success of single-cell proteomics, and this study proposes a microfluidics-based method for highly efficient isolation, detection, and collection of rare cells such as CTCs, providing an effective handling system for subsequent proteomic analysis.
Advancements in single-cell-related technologies have opened new possibilities for analyzing rare cells, such as circulating tumor cells (CTCs) and rare immune cells. Among these techniques, single-cell proteomics, particularly single-cell mass spectrometric analysis (scMS), has gained significant attention due to its ability to directly measure transcripts without the need for specific reagents. However, the success of single-cell proteomics relies heavily on efficient sample preparation, as protein loss in low-concentration samples can profoundly impact the analysis. To address this challenge, an effective handling system for rare cells is essential for single-cell proteomic analysis. Herein, we propose a microfluidics-based method that offers highly efficient isolation, detection, and collection of rare cells (e.g., CTCs). The detailed fabrication process of the micropillar array-based microfluidic device is presented, along with its application for CTC isolation, identification, and collection for subsequent proteomic analysis.
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