Journal
NEUROSCIENCE RESEARCH
Volume 110, Issue -, Pages 59-67Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neures.2016.04.001
Keywords
Orexin-A; Hippocampus; The perifornical area; Gastric distension responsive neurons; Gastric motility; Electrophysiological experiment
Categories
Funding
- National Natural Science Foundation of China [81470815, 31071014, 81100260, 81270460, 81300281, 81500414]
- Research Award Fund for Outstanding Middle-aged and Young Scientist of Shandong Province [BS2014YY009]
- Shandong Province Natural Science Foundation [ZR2014CM014]
- Qingdao Municipal Science and Technology Commission [13-1-4-170-jch, 14-2-3-3-nsh]
Ask authors/readers for more resources
Orexin-A is mainly produced in the lateral hypothalamus (LHA) and the perifornical area (PeF). Here, we aim to elucidate the effects of orexin-A in the hippocampus (Hi) on gastric distention (GD)-sensitive neurons and gastric motility, and potential regulation mechanisms by the PeF. Retrograde tracing and fluorescent-immunohistochemical staining were used to determine orexin-A neuronal projections. Single unit discharges in the Hi were recorded extracellularly and gastric motility in conscious rats was monitored during administration of orexin-A to the Hi or electrical stimulation of the PeF. Orexin-A administration to the Hi excited most of the GD-excitatory (GD-E) neurons and GD-inhibitory (GD-I) neurons, and increased gastric motility in a dose-dependent manner. All of effects induced by orexin-A could be partly blocked by pretreatment with orexin-A antagonist, SB-334867. Electrical stimulation of the PeF excited the majority of the orexin-A-responsive GD neurons in the Hi and promoted gastric motility. Additionally, pretreatment with SB-334867 in the Hi increased the firing rate of GDI and GDE neurons following electrical stimulation of the PeF. These findings suggest that orexin-A could regulate activities of GD-sensitive neurons and gastric motility. Furthermore, the PeF may be involved in this regulatory pathway. (C) 2016 Elsevier Ireland Ltd and Japan Neuroscience Society. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available