Journal
CHEMICO-BIOLOGICAL INTERACTIONS
Volume 233, Issue -, Pages 8-13Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2015.03.014
Keywords
Lung cancers; Apoptosis; A549; 4-O-(2 ''-O-acetyl-6 ''-O-p-coumaroyl-beta-D-glucopyranosyl)-p-coumaric acid
Funding
- developmental fund of Chen Keji Integrative Medicine [CKJ2010026]
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This study was aimed to investigate antitumor activity of 4-O-(2-O-acetyl-6-O-p-coumaroyl-beta-D-glucopyranosyl)-p-coumaric acid (4-ACGC) against lung cancer and its mechanisms. The anti-proliferative effects of 4-ACGC on lung cancer cell lines including A549, NCI-H1299, HCC827 were evaluated by MIT method and the IC50 values were calculated, and subsequently a mice xenograft model of A549 was established to investigate the antitumor effect of 4-ACGC in vivo. Furthermore, the apoptosis of the A549 cells was determined by fluorescence microscope by staining with Hoechst 33324 and flow cytometer by staining with FITC conjugated Annexin V/PI, and the further mechanisms were investigated by Western blotting. Our results demonstrated that 4-ACGC possessed notable anti-tumor activity on lung cancer in vivo and in vitro; the mechanisms were involved in inducing mitochondria-mediated apoptosis via up-regulations of caspase-3, caspase-9, Bad and Bax, and down-regulation of Bcl-2. Collectively, our results indicated that the 4-ACGC could be treated as a new candidate for treatment of lung cancer in the future. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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