Journal
NEUROSCIENCE LETTERS
Volume 631, Issue -, Pages 13-18Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2016.08.010
Keywords
Brain imaging; Cells specific dye; Astrocyte; Thyroid hormone transporter; Gap-junctions
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Funding
- Deutsche Forschungsgemeinschaft (DFG) [HU787/8-1]
- Cluster of Excellence
- DFG Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)
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The red fluorescent dye Sulforhodamine 101 (SR101) is often used as a marker for astrocytes, although variations of the staining protocol have been shown to influence the preferentially labeled cell type. Here we analyzed SR101-labeling of oligodendrocytes in the hippocampal slices preparation of PLP-EGFP mice. Using different staining protocols, we found robust SR101-labeled oligodendrocytes in the CA1 stratum radiatum of the hippocampus. Application of L-thyroxin, which is known to block SR101 transport into astrocytes via competitive inhibition of the multi-specific OATP1C1 (SLCO1C1) transporter, significantly reduced oligodendrocyte labeling. Since OATP1C1 is not expressed in oligodendrocytes, we conclude that oligodendrocyte labeling with SR101 requires SR101-uptake by astrocytes, which then diffuses to oligodendrocytes via heterotypic gap junctions of the pan-glial network. In summary, unequivocal identification of a particular cell type is not possible by SR101 only, hence caution is recommended when using SR101 in future studies. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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