Journal
CURRENT OPINION IN BIOMEDICAL ENGINEERING
Volume 27, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.cobme.2023.100471
Keywords
CRISPR Pathogen detection Multiplexing Point-of-care Nucleic acid; amplification; CRISPR; Pathogen detection; Multiplexing Point-of-care; Nucleic acid amplification
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Bacterial and viral pathogens pose serious threats to human health. Multiplexed nucleic acid detection methods, especially CRISPR-based methods, have shown great potential in detecting multiple species and variants of pathogens. This review provides an overview of current multiplexed nucleic acid detection methods, focusing on CRISPR-based methods, and discusses the future of multiplexed point-of-care diagnostics.
Bacterial and viral pathogens are devastating to human health and well-being. In many regions, dozens of pathogen species and variants co-circulate. Thus, it is important to detect many different species and variants of pathogens in a given sample through multiplexed detection methods. CRISPR-based nucleic acid detection has shown to be a promising step towards an easy-to-use, sensitive, specific, and high-throughput method to detect nucleic acids from DNA and RNA viruses and bacteria. Here, we review the current state of multiplexed nucleic acid detection methods with a focus on CRISPR-based methods. We also look toward the future of multiplexed pointof-care diagnostics.
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