Journal
NEUROSCIENCE LETTERS
Volume 618, Issue -, Pages 139-145Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2016.02.054
Keywords
Autophagy; Beclin-1; Hyperbaric oxygen; LC3; Spinal cord injury
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Funding
- Outstanding Medical Professional Award from the Suzhou City Government (Suzhou, China) [RC0802]
- 333 project of Jiangsu province (Jiangsu, China) [BRA2011047]
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The purpose of this study was to explore the effects of Hyperbaric oxygen (HBO) on the autophagic changes after induction of spinal cord injury (SCI) in rats. A total of 75 rats were randomly divided into the sham-operated group, the spinal cord injury group, and the SCI + HBO group. We found that at 7 d and 14 d after surgery, the BBB scores were higher in the SCI + HBO group in comparison to the SCI group. The expression of Beclin-1 and LC3II was upregulated in the SCI and SCI + HBO groups after SCI. Fluorescently stained Beclin-1 and LC3II proteins were barely detectable in the sham group. In contrast, Beclin-1 and LC3II expression was observed in neurons and glial cells from the SCI and SCI + HBO groups. Beclin-1 and LC3II expression appeared at 6 h after SCI. At each time point, Beclin-1 and LC3II expression was significantly higher in the SCI + HBO group compared to the SCI group. These results suggest that autophagy is activated in rats after SCI and sustained over a period of time. HBO treatment enhances autophagy expression in rats after SCI and accelerates cell repair rate, which may represent one of the mechanisms of action of HBO in the treatment of SCI. (C) 2016 The Authors. Published by Elsevier Ireland Ltd.
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