Journal
BIOCHEMISTRY AND BIOPHYSICS REPORTS
Volume 34, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbrep.2023.101448
Keywords
Hypochlorous acid; Hyaluronan; Glycosaminoglycans; Monosaccharides; ESI MS; NMR spectroscopy; UV spectroscopy
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Hypochlorous acid (HOCl), generated by myeloperoxidase during inflammation, is a strong oxidant that reacts with sulfhydryl and amino acid residues. It also affects other biomolecules, such as glycosaminoglycans. This study investigates the degradation of glycosaminoglycans by HOCl and the conversion of related monosaccharides into chlorinated products using mass spectrometry. The reaction between HOCl and hyaluronan is slower than previously thought, resulting in the cleavage of beta-1,3/1,4-glycosidic linkages and the generation of different products, particularly hyaluronan monosaccharides. The detection of chlorinated products is only possible for monosaccharides, and potential reasons for this will be discussed.
Hypochlorous acid (HOCl) is a strong non-radical oxidant, which is generated during inflammatory processes under the catalysis of the enzyme myeloperoxidase (MPO). HOCl reacts particularly with sulfhydryl and amino acid residues but affects also many other biomolecules. For instance, the glycosaminoglycans of articular cartilage and synovial fluids (such as hyaluronan) undergo degradation in the presence of HOCl at which the native polysaccharide is fragmented into oligosaccharides in a complex reaction. This is an initial mass spectrometry (MS)-based investigation dealing with the HOCl-induced degradation of glycosaminoglycans and the conversion of the related monosaccharides into chlorinated products. In particular, it will be shown that the reaction between HOCl and hyaluronan is slower than originally assumed and results in the generation of different products (particularly the hyaluronan monosaccharides) by the cleavage of the beta-1,3/ 1,4-glycosidic linkages. The MS detection of chlorinated products is, however, only possible in the case of the monosaccharides. Potential reasons will be discussed.
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