Journal
NEUROPHARMACOLOGY
Volume 100, Issue -, Pages 76-89Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2015.07.017
Keywords
BONLAC; BONCAT; SILAC; de nova proteomics; BDNF; hippocampal slice; synaptic plasticity; protein synthesis
Categories
Funding
- NIH [T32 MH019524, NS034007, NS047384, NS21072, HD23315, NS050276, RR027990, GM113237]
- Simons Foundation [274864]
- NSF [CHE-1152317]
- FRAXA Research Foundation
- Charles H. Revson Senior Fellowship in Biomedical Science
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1152317] Funding Source: National Science Foundation
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Stimulus-triggered protein synthesis is critical for brain health and function. However, due to technical hurdles, de novo neuronal translation is predominantly studied in cultured cells, whereas electrophysiological and circuit analyses often are performed in brain slices. The different properties of these two experimental systems create an information gap about stimulus-induced alterations in the expression of new proteins in mature circuits. To address this, we adapted two existing techniques, BONCAT and SILAC, to a combined proteomic technique, BONLAC, for use in acute adult hippocampal slices. Using BDNF-induced protein synthesis as a proof of concept, we found alterations in expression of proteins involved in neurotransmission, trafficking, and cation binding that differed from those found in a similar screen in cultured neurons. Our results indicate important differences between cultured neurons and slices, and suggest that BONLAC could be used to dissect proteomic changes underlying synaptic events in adult circuits. This article is part of the Special Issue entitled 'Synaptopathy - from Biology to Therapy'. (C) 2015 Elsevier Ltd. All rights reserved.
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