Association of Kruppel-like factor 2 polymorphisms with the risk of coronary artery disease

Purpose: Coronary artery disease is defined as complications that develop in proportion to the prevalence of ischemia due to occlusion of the coronary arteries and the resulting cell death. The development of atherosclerosis is significantly influenced by endothelial cell dysfunction. Kruppel-like factor 2, a transcription factor, has been shown to regulate critical biological events in endothelial biology, such as vascular tone, migration, proliferation, vasoreactivity, and angiogenesis. In our study, it was aimed to clarify the relationship between coronary artery disease and Kruppel-like factor 2 protein levels and C1239A polymorphisms.Materials and Methods: 191 individuals who underwent coronary angiography at Mersin University Faculty of Medicine, Department of Cardiology, Health, Research and Application Center were included in the study. Measurements of serum fasting blood glucose, HDL cholesterol, total cholesterol, and triglyceride levels were performed on the AU5800 (Beckman Coulter, United States) autoanalyzer. Serum LDL levels were calculated using the Friedwald equation. Serum Kruppel-like factor 2 protein levels were measured by sandwich enzyme-linked immunoassay method on the Multiskan GO (Thermo Scientific, Finland) device. Kruppel-like factor 2 C1239A variations were detected on the Applied Biosystem VIIATM 7 Real-Time PCR (Life Technologies Co., United States) device by TaqMan (R) single nucleotide polymorphism (SNP) genotyping method.Results: Men had a 3.8-fold higher risk of CAD than women. (Odd's ratio 3.83, 95% Confidence interval 1.98- 7.39; p<0.001). Kruppel-Like Factor 2 protein levels werenot significantly different amongst the groups. Kruppel-like factor 2 C1239A polymorphisms were not associated with coronary artery disease.Conclusion: More comprehensive studies with larger sample groups including other gene regions and meta -analysis studies are needed to determine the relationship between Kruppel-like factor 2 gene polymorphisms and coronary artery disease.

Automated summary

This study aimed to clarify the relationship between coronary artery disease and Kruppel-like factor 2 protein levels and C1239A polymorphisms. The results showed that men had a 3.8-fold higher risk of CAD than women, but Kruppel-like factor 2 protein levels and C1239A polymorphisms were not associated with coronary artery disease.