Journal
NEUROCHEMICAL RESEARCH
Volume 41, Issue 8, Pages 1877-1886Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-016-1899-8
Keywords
Stroke; Sympathetic nervous system; Immunosuppression; beta-Arrestin2; Nuclear factor-kappa B
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Funding
- National Nature Science Foundation of China [81271336]
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Stoke results in activation of the sympathetic nervous system (SNS), inducing systemic immunosuppression. However, the potential mechanisms underlying stroke-induced immunosuppression remain unclear. Here, we determined the SNS effects on functional outcome and explored the interactions among SNS, beta-arrestin2 and nuclear factor-kappa B (NF-kappa B) after experimental stroke in rats. In the current study, stroke was induced by a transient middle cerebral artery occlusion (MCAO) in rats, and SNS activity was inhibited by intraperitoneal injection of 6-hydroxydopamine HBr (6-OHDA). 7.0 T Micro-MRI and Longa score were employed to assess the functional outcome after stroke. Flow cytometry and ELISA assay were used to measure the expression of MHC class II, tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma). Western blot was conducted to analyze beta-arrestin2 and NF-kappa B protein expression levels after experimental stroke. We found significantly increased infarct volumes and functional impairment after MCAO at different post-surgery time points, which were not aggravated by 6-OHDA treatment. SNS blockade partially reversed the expression of MHC class II after stroke over time, as well as TNF-alpha and IFN-gamma levels in lipopolysaccharide-stimulated macrophages in vitro. Treatment of MCAO rats with SNS-inhibitor significantly diminished NF-kappa B activation and enhanced beta-arrestin2 expression after stroke. This study suggests that pharmacological SNS inhibition dose not aggravate functional outcome after stroke. Stroke-induced immunosuppression may be involved in the SNS-beta-arrestin2-NF-kappa B pathway.
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