Anti-inflammatory of disenecionyl cis-khellactone in LPS-stimulated RAW264.7 cells and the its inhibitory activity on soluble epoxide hydrolase

The objective of the present study was to investigate anti-inflammatory effects of disenecionyl ciskhellactone (DK) isolated from Peucedanum japonicum Thunberg, a traditional edible plant, in RAW264.7 cells stimulated with lipopolysaccharide (LPS). Anti-inflammatory effects of DK were analyzed using various techniques, including NO assay, Western blot analysis, enzyme-linked immunosorbent assay (ELISA), real-time PCR, and immunofluorescence staining. It was revealed that DK reduced the production of pro-inflammatory cytokines including Monocyte chemoattractant protein-1 (MCP-1), Tumor necrosis factor-alpha (TNF-alpha), Interleukin 1 beta (IL-1 beta), and Interleukin 6 (IL-6) in RAW264.7 cells stimulated with LPS. It was revealed that DK effectively downregulated expression levels of iNOS and COX-2 due to inhibition of NF-kappa B activation and suppressing the phosphorylation of p38 and jun N-terminal kinase (JNK) mitogen-activated protein kinase (MAPK) phosphorylation. Also, soluble epoxide hydrolase activity and expression were decreased by the proinflammatory inhibitor, DK. Finally, findings of this study suggest that DK isolated from P. japonicum might have potential as a therapeutic candidate for inflammatory diseases.

Automated summary

In this study, the anti-inflammatory effects of DK extracted from Peucedanum japonicum Thunberg were investigated. The results showed that DK reduced the production of pro-inflammatory cytokines and downregulated the expression levels of iNOS and COX-2. DK also inhibited NF-kappa B activation and suppressed the phosphorylation of p38 and JNK MAPK phosphorylation. Furthermore, DK decreased the activity and expression of soluble epoxide hydrolase. These findings suggest that DK could be a potential candidate for the treatment of inflammatory diseases.