Journal
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
Volume 8, Issue 3, Pages 452-461Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.synbio.2023.06.006
Keywords
Cytochrome P450 enzymes; P450 BM3 peroxygenase; Indigoids; Dyestuff; Organic electronics
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In this study, cytochrome P450 BM3 was engineered into peroxygenases through directed evolution. These modified enzymes were used to selectively oxidize indole derivatives to produce a variety of indigoid pigments with a reduced band gap. These indigoid photocatalysts show promise for the development of new organic electronic devices.
Indigoids, a class of bis-indoles, have long been applied in dyeing, food, and pharmaceutical industries. Recently, interest in these 'old' molecules has been renewed in the field of organic semiconductors as functional building blocks for organic electronics due to their excellent chemical and physical properties. However, these indigo derivatives are difficult to access through chemical synthesis. In this study, we engineer cytochrome P450 BM3 from an NADPH-dependent monooxygenase to peroxygenases through directed evolution. A select number of P450 BM3 variants are used for the selective oxidation of indole derivatives to form different indigoid pigments with a spectrum of colors. Among the prepared indigoid organic photocatalysts, a majority of indigoids demonstrate a reduced band gap than indigo due to the increased light capture and improved charge separation, making them promising candidates for the development of new organic electronic devices. Thus, we present a useful enzymatic approach with broad substrate scope and cost-effectiveness by using low-cost H2O2 as a cofactor for the preparation of diversified indigoids, offering versatility in designing and manufacturing new dyestuff and electronic/sensor components.
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