Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering

Restriction endonucleases are a component of restriction-modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules. There are four main types of restriction enzymes (types I, II, III, and IV), which differ in protein composition, cofactor requirements, and mode of action. The most studied are representatives of type II, which specifically recognize DNA sequences of 4-8 bp and catalyze DNA cleavage within these sequences or not far from them. The exceptional precision of type II enzymes has made them indispensable for DNA manipulations. Although hundreds of DNA restriction enzymes are currently known, there is still a need for enzymes that recognize new DNA targets. For this reason, the discovery of new natural restriction endonucleases and rational design of their properties (to obtain enzymes with high specificity for a unique nucleotide sequence at a restriction site and without nonspecific activity) will expand the list of enzymes for use in biotechnology and genetic engineering. This review briefly touches upon the main types of restriction endonucleases, their classification, nomenclature, and typical properties, and it concisely describes approaches to the construction of enzymes with altered properties.

Automated summary

Restriction endonucleases are important components of restriction-modification systems in bacteria, providing protection against foreign DNA. Type II restriction enzymes, which specifically recognize and cleave DNA sequences of 4-8 bp, are the most extensively studied. The discovery and design of new restriction endonucleases with high specificity and unique properties will expand the applications of biotechnology and genetic engineering.