Model-based assessment of neutrophil-mediated phagocytosis and digestion of bacteria across in vitro and in vivo studies

Neutrophil granulocytes are key components of the host response against pathogens, and severe neutropenia, with neutrophil counts below 0.5 x 10(6) cells/mL, renders patients increasingly vulnerable to infections. Published in vitro (n = 7) and in vivo (n = 5) studies with time-course information on bacterial and neutrophil counts were digitized to characterize the kinetics of neutrophil-mediated bacterial killing and inform on the immune systems' contribution to the clearance of bacterial infections. A mathematical model for the in vitro dynamics of bacteria and the kinetics of neutrophil-mediated phagocytosis and digestion was developed, which was extended to in vivo studies in immune-competent and immune-compromised mice. Neutrophil-mediated bacterial killing was described by two first-order processes-phagocytosis and digestion-scaled by neutrophil concentration, where 50% of the maximum was achieved at neutrophil counts of 1.19 x 10(6) cells/mL (phagocytosis) and 6.55 x 10(6) cells/mL (digestion). The process efficiencies diminished as the phagocytosed bacteria to total neutrophils ratio increased (with 50% reduction at a ratio of 3.41). Neutrophil in vivo dynamics were captured through the characterization of myelosuppressive drug effects and postinoculation neutrophil influx into lungs and by system differences (27% bacterial growth and 9.3% maximum capacity, compared with in vitro estimates). Predictions showed how the therapeutically induced reduction of neutrophil counts enabled bacterial growth, especially when falling below 0.5 x 10(6) cells/mL, whereas control individuals could deal with all tested bacterial burdens (up to 10(9) colony forming units/g lung). The model-based characterization of neutrophil-mediated bacterial killing simultaneously predicted data across in vitro and in vivo studies and may be used to inform the capacity of host-response at the individual level.

Automated summary

Neutrophil granulocytes play a crucial role in the host's immune response against pathogens, and severe neutropenia can make individuals more vulnerable to infections. This study developed a mathematical model to characterize the kinetics of neutrophil-mediated bacterial killing and investigated the contribution of the immune system to the clearance of bacterial infections. The model successfully predicted data from both in vitro and in vivo studies and can be used to assess the capacity of the host immune response at an individual level.