Salt Stress-Related Mechanisms in Leaves of the Wild Barley Hordeum spontaneum Generated from RNA-Seq Datasets

This study aims to detect salt stress-related genes and mechanisms of the wild barley Hordeum spontaneum. Among the generated RNA-Seq datasets, several regulated transcripts are influenced by levels of cellular carbon, nitrogen and oxygen. Some of the regulated genes act on photorespiration and ubiquitination processes, as well as promoting plant growth and development under salt stress. One of the genes, encoding alanine:glyoxylate aminotransferase (AGT), participates in signaling transduction and proline biosynthesis, while the gene encoding asparagine synthetase (ASN) influences nitrogen storage and transport in plants under stress. Meanwhile, the gene encoding glutamate dehydrogenase (GDH) promotes shoot and root biomass production as well as nitrate assimilation. The upregulated genes encoding alpha-aminoadipic semialdehyde synthase (AASAS) and small auxin-up RNA 40 (SAUR40) participate in the production of proline and signaling compounds, respectively, while the gene encoding E3 ubiquitin-protein ligase regulates the carbon/nitrogen-nutrient response and pathogen resistance, in addition to some physiological processes under biotic and abiotic stresses via signal transduction. The gene encoding the tetratricopeptide repeat (TPR)-domain suppressor of STIMPY (TSS) negatively regulates the carbon level in the cell. In conclusion, this study sheds light on possible molecular mechanisms underlying salt stress tolerance in wild barley that can be utilized further in genomics-based breeding programs of cultivated species.

Automated summary

The study aims to identify salt stress-related genes and mechanisms in wild barley Hordeum spontaneum. Analysis of RNA-Seq datasets revealed that several regulated transcripts are influenced by cellular levels of carbon, nitrogen, and oxygen. These regulated genes are involved in processes such as photorespiration, ubiquitination, plant growth, and development under salt stress. The study identifies specific genes encoding alanine:glyoxylate aminotransferase (AGT), asparagine synthetase (ASN), glutamate dehydrogenase (GDH), alpha-aminoadipic semialdehyde synthase (AASAS), small auxin-up RNA 40 (SAUR40), E3 ubiquitin-protein ligase, and tetratricopeptide repeat (TPR)-domain suppressor of STIMPY (TSS) that play important roles in various cellular and physiological processes under biotic and abiotic stresses.