Co-Activation of Human Whole Blood Cells with Lipopolysaccharides and an Allergen

The investigation of common inflammation mechanisms caused by exogenic compounds of microbial origin and allergens is one of the most important tasks in current biomedical science. The main manifestations of immune cell activation caused by pro-inflammatory agents are changes in receptor quantity on the surface of immune cells and the production of cytokines and chemokines by blood cells. The levels of expression of TLR4, CD14, and CD11b in the monocytes and neutrophils of human whole blood in response to LPS E. coli, Der p 2 allergen, or their combination reflect different functional activities in these cells, while the composition and amount of produced cytokines reflect the biological activity of the studied agonists. The activity of Der p 2 allergen in ex vivo experiments on whole blood samples is significantly lower compared with its activity in vitro in isolated PBMC cells, which should be taken into account when transferring the results obtained for isolated cells to whole blood cells. LPS R. capsulatus PG significantly decreases the synthesis of MyD88-dependent NF-kappa B-regulated cytokines activated by LPS E. coli, Der p 2, or their combination. This indirectly indicates the general mechanisms of cell activation caused by these structures and the unified mechanism of the protective action of LPS R. capsulatus PG against both endotoxin and a combination of endotoxin and the allergen.

Automated summary

The investigation of common inflammation mechanisms caused by exogenic compounds of microbial origin and allergens is crucial in biomedical science. The activation of immune cells by pro-inflammatory agents leads to changes in receptor quantity and cytokine production by blood cells. The expression levels of TLR4, CD14, and CD11b in monocytes and neutrophils reflect their functional activities, while the composition and amount of cytokines produced reflect the biological activity of the studied agonists. Furthermore, the activity of Der p 2 allergen in whole blood samples is lower compared to isolated PBMC cells, and LPS R. capsulatus PG reduces the synthesis of cytokines activated by LPS and Der p 2, suggesting a unified mechanism of cell activation and protective action.