Quantitative Detection of the Influenza a Virus by an EGOFET-Based Portable Device

Elaboration of biosensors on the base of organic transistors with embedded biomolecules which can operate in an aqueous environment is of paramount importance. Electrolyte-gated organic field-effect transistors demonstrate high sensitivity in detection of various analytes. In this paper, we demonstrated the possibility of quantitative fast specific determination of virus particles by an aptasensor based on EGOFET. The sensitivity and selectivity of the devices were examined with the influenza A virus as well as with control bioliquids like influenza B, Newcastle disease viruses or allantoic fluid with different dilutions. The influence of the semiconducting layer thickness on EGOFETs sensory properties is discussed. The fabrication of a multi-flow cell that simultaneously registers the responses from several devices on the same substrate and the creation of a multi-sensor flow device are reported. The responses of the elaborated bioelectronic platform to the influenza A virus obtained with application of the portable multi-flow mode are well correlated with the responses obtained in the laboratory stationary mode.

Automated summary

In this paper, the possibility of fast quantitative specific determination of virus particles using EGOFET-based aptasensors in an aqueous environment was demonstrated. The sensitivity and selectivity of the devices were examined using various analytes including influenza A and B viruses, Newcastle disease viruses, and allantoic fluid. The effects of the thickness of the semiconducting layer on the sensory properties of EGOFETs were discussed, and the fabrication of a multi-flow cell and a multi-sensor flow device were reported. The responses obtained with the portable multi-flow mode were well correlated with those obtained in the laboratory stationary mode.