Journal
BIOSENSORS-BASEL
Volume 13, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/bios13080768
Keywords
reagent delivery; nanodroplet; microdroplet; biosensor; microfluidic device
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In vitro compartmentalization (IVC) is a technique used to establish the genotype-phenotype linkage, and fluorinated oils have become more widely used in making microdroplets. However, performing multi-step reactions in water-in-fluorinated-oil microdroplets is difficult. To overcome this issue, a nanodroplet-based approach for the delivery of copper ions and middle-sized peptide molecules has been demonstrated.
In vitro compartmentalization (IVC) is a technique for generating water-in-oil microdroplets to establish the genotype (DNA information)-phenotype (biomolecule function) linkage required by many biological applications. Recently, fluorinated oils have become more widely used for making microdroplets due to their better biocompatibility. However, it is difficult to perform multi-step reactions requiring the addition of reagents in water-in-fluorinated-oil microdroplets. On-chip droplet manipulation is usually used for such purposes, but it may encounter some technical issues such as low throughput or time delay of reagent delivery into different microdroplets. Hence, to overcome the above issues, we demonstrated a nanodroplet-based approach for the delivery of copper ions and middle-sized peptide molecules (human p53 peptide, 2 kDa). We confirmed the ion delivery by microscopic inspection of crystal formation inside the microdroplet, and confirmed the peptide delivery using a fluorescent immunosensor. We believe that this nanodroplet-based delivery method is a promising approach to achieving precise control for a broad range of fluorocarbon IVC-based biological applications, including molecular evolution, cell factory engineering, digital nucleic acid detection, or drug screening.
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