Spatial Omics Sequencing Based on Microfluidic Array Chips

Spatial profiling technologies fill the gap left by the loss of spatial information in traditional single-cell sequencing, showing great application prospects. After just a few years of quick development, spatial profiling technologies have made great progress in resolution and simplicity. This review introduces the development of spatial omics sequencing based on microfluidic array chips and describes barcoding strategies using various microfluidic designs with simplicity and efficiency. At the same time, the pros and cons of each strategy are compared. Moreover, commercialized solutions for spatial profiling are also introduced. In the end, the future perspective of spatial omics sequencing and research directions are discussed.

Automated summary

Spatial profiling technologies have promising application prospects in filling the gap left by the loss of spatial information in traditional single-cell sequencing. Within a few years of development, these technologies have made significant progress in resolution and simplicity. This review discusses the development of spatial omics sequencing based on microfluidic array chips, barcoding strategies using various microfluidic designs, and the pros and cons of each strategy. Commercialized solutions for spatial profiling and future research directions are also presented.