Journal
BIOSENSORS-BASEL
Volume 13, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/bios13110955
Keywords
Ochratoxin A; PAMAM; electrochemical aptasensor; graphene oxide; AuNPs
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In this study, an electrochemical aptasensor using PAMAM dendrimers as a signal amplifier was developed for the sensitive detection of OTA. The aptasensor showed high sensitivity, specificity, and a broad linear detection range with a low detection limit. The proposed aptasensor was successfully applied for OTA analysis in red wine and demonstrated good recovery rates and storage stability.
Sensitive detection of ochratoxin A (OTA) is significant and essential because OTA may pose risks to human and animal health. Here, we developed an electrochemical aptasensor for OTA analysis using polyamidoamine (PAMAM) dendrimers as a signal amplifier. As a carrier, PAMAM has numerous primary amino groups that can be coupled with thiolated complementary strand DNA (cDNA), allowing it to recognize aptamers bound to the surface of horseradish peroxidase (HRP)-modified gold nanoparticles (AuNPs), thereby improving the sensitivity of the aptasensor. When monitoring the positive samples, OTA was captured by the aptamer fixed on the HRP-conjugated AuNP surface by specific recognition, after which the formed OTA-aptamer conjugates were detached from the electrode surface, ultimately decreasing the electrochemical signal monitored by differential pulse voltammetry. The novel aptasensor achieved a broad linear detection range from 5 to 105 ng L-1 with a low detection limit of 0.31 ng L-1. The proposed aptasensor was successfully applied for OTA analysis in red wine, with recovery rates ranging from 94.15 to 106%. Furthermore, the aptasensor also exhibited good specificity and storage stability. Therefore, the devised aptasensor represents a sensitive, practical and reliable tool for monitoring OTA in agricultural products, which can also be adapted to other mycotoxins.
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