Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests

Article Medical Laboratory Technology

Evaluation of the Visby medical COVID-19 point of care nucleic acid amplification test

Brooke M. Katzman et al.

Summary: The Visby Medical COVID-19 test is a rapid and accurate method for detecting SARS-CoV-2. It has high concordance with laboratory-based reference methods and superior sensitivity compared to other point-of-care devices.

CLINICAL BIOCHEMISTRY (2023)

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Article Biochemistry & Molecular Biology

Development of Reverse Transcription Recombinase Polymerase Amplification (RT-RPA): A Methodology for Quick Diagnosis of Potato Leafroll Viral Disease in Potato

Ravinder Kumar et al.

Summary: Potato virus transmission is always possible due to the vegetative development of potatoes from tubers. The potato leafroll virus (PLRV) is a highly destructive virus and is considered the second-most destructive virus after Potato virus Y. Multiple aphid species transmit PLRV, causing significant yield and quality reduction. The RT-RPA method was used to detect PLRV in both potato tubers and leaves with high sensitivity and efficiency, making it a valuable tool for virus detection and monitoring.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

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Article Plant Sciences

Rapid and Sensitive Detection of Verticillium dahliae from Complex Samples Using CRISPR/Cas12a Technology Combined with RPA

Qiang Wang et al.

Summary: Verticillium wilt, caused by Verticillium dahliae, is a significant soilborne plant disease worldwide. In this study, a detection method using CRISPR/Cas12a and RPA technologies was developed for accurate and rapid detection of V. dahliae. The detection system had a lower detection limit of approximately 10 copies of genomic DNA per reaction and could detect as little as one microsclerotium per gram of soil. This study expands the application of CRISPR/Cas12a nucleic acid detection to soilborne crop diseases and contributes to the development of field-deployable diagnostic tools.

PLANT DISEASE (2023)

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Article Materials Science, Multidisciplinary

Paper-Based Bacterial Lysis Enables Sample-to-Answer Home-based DNA Testing

Georgios Chondrogiannis et al.

Summary: Nucleic acid amplification testing (NAAT) is the gold standard for infectious disease diagnostics, but it is mainly limited to centralized laboratories. Researchers have addressed the bottleneck issue of DNA extraction by immobilizing the enzyme ACP on nitrocellulose, enabling home-based kits and expanding the capabilities of home-testing beyond antigen tests.

ADVANCED MATERIALS TECHNOLOGIES (2023)

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Article Microbiology

Rapid and Visual Detection of SARS-CoV-2 RNA Based on Reverse Transcription-Recombinase Polymerase Amplification with Closed Vertical Flow Visualization Strip Assay

Yumeng Song et al.

Summary: Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, emerged in 2019 and rapidly spread worldwide. As the epidemic progressed, new variants with higher transmission speeds and infectivity emerged. The proportions of asymptomatic infections and reinfections after vaccination have increased, making prevention and control extremely difficult. A nucleic acid visualization assay targeting the SARS-CoV-2 nucleoprotein (N) gene was developed, which showed high sensitivity and specificity and could detect multiple SARS-CoV-2 variants within 25 minutes.

MICROBIOLOGY SPECTRUM (2023)

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Article Veterinary Sciences

Development of a multienzyme isothermal rapid amplification and lateral flow dipstick combination assay for bovine coronavirus detection

Chengyuan Ji et al.

Summary: In this study, a novel multienzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD) combination assay were developed for rapid on-site detection of Bovine coronavirus (BCoV) infection. The assay was highly specific and sensitive, comparable to a published reverse transcription quantitative PCR (RT-qPCR) assay for BCoV detection. It did not require sophisticated instruments and the results could be observed with eyes.

FRONTIERS IN VETERINARY SCIENCE (2023)

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Article Nanoscience & Nanotechnology

Ultrasensitive Detection of miRNA via CRISPR/Cas12a Coupled with Strand Displacement Amplification Reaction

Shaoqiong Feng et al.

Summary: A distance-based biosensor using hydrogel, CRISPR/Cas12a and target-triggered strand displacement amplification (SDA) reaction is proposed for the visual, quantitative and sensitive measurement of microRNA. The biosensor can detect the concentration of target microRNA visually without the assistance of instruments, and it shows high sensitivity and specificity, making it suitable for point-of-care applications.

ACS APPLIED MATERIALS & INTERFACES (2023)

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Article Chemistry, Analytical

A Collection of RPA-Based Photoelectrochemical Assays for the Portable Detection of Multiple Pathogens

Rui Ge et al.

Summary: In this study, a photoelectrochemical (PEC) biosensor combined with recombinase polymerase amplification (RPA) technology (RPA-PEC) was proposed for rapid detection of multiple foodborne pathogens. The RPA was initiated on homemade three-dimensional screen-printed paper-based electrodes, forming a lab-on-paper platform. Using the DNA-PEC signaler, photocurrents were achieved after only 20 minutes of RPA, with superior detection performance compared to conventional agarose gel electrophoresis.

ANALYTICAL CHEMISTRY (2023)

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Article Chemistry, Analytical

Thermus thermophilus Argonaute-Based Isothermal Amplification Assay for Ultrasensitive and Specific RNA Detection

Changjing Yuan et al.

Summary: Recent advances in prokaryotic Argonaute proteins (pAgos) have provided new insights into the development of pAgos-based nucleic acid detection platforms. However, the current challenge lies in achieving isothermal detection using pAgos. This study introduces a true isothermal amplification strategy called Thermus thermophilus Argonaute-based thermostable exponential amplification reaction (TtAgoEAR), which allows for RNA detection with ultra-sensitivity and single-nucleotide resolution at a constant temperature of 66 degrees C. The results demonstrate the potential of TtAgoEAR for reliable and easy RNA detection in point-of-care diagnosis and field analysis.

ANALYTICAL CHEMISTRY (2023)

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Article Biochemical Research Methods

Paper-Based Radial Flow Assay Integrated to Portable Isothermal Amplification Chip Platform for Colorimetric Detection of Target DNA

Tai-Yong Kim et al.

Summary: A novel integrated detection system was developed for on-site colorimetric detection of DNA, by introducing a paper-chip-based molecular detection strategy into a PDMS microchip and temperature control system. The system optimized the rolling circle amplification reaction for signal amplification and the Au-probe labeling strategy for visualization. The PDMS chip allowed one-step reactions for ligation, amplification, and labeling under isothermal temperature, and the final reaction solution was loaded onto the paper chip to generate a radial colorimetric signal. The system achieved a detection limit of 14.7 nM of DNA and selectively discriminated non-specific DNAs with a single-base mismatch. It has potential applications in single nucleotide polymorphism identification and pathogen gene detection due to its cost-effectiveness, portability, and user-friendliness in various resource-limited locations.

BIOCHIP JOURNAL (2023)

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Article Biophysics

An ultra-sensitive one-pot RNA-templated DNA ligation rolling circle amplification-assisted CRISPR/Cas12a detector assay for rapid detection of SARS-CoV-2

Zaobing Zhu et al.

Summary: The article introduces a sensitive and rapid diagnosis method called OPERATOR, which utilizes enzyme-catalyzed rolling circle amplification-assisted CRISPR/FnCas12a assay for detecting SARS-CoV-2. OPERATOR presents advantages of ultra-sensitivity, high specificity, rapid reaction speed, easy operation, low cost, and on-spot visualization. It can be combined with rapid RNA release and a lateral flow strip for point-of-care testing.

BIOSENSORS & BIOELECTRONICS (2023)

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Article Agronomy

Development of a new PCR assay and a recombinase-aided amplification based isothermal amplification coupled with lateral flow dipstick assay for potato late blight detection

Xue Tang et al.

Summary: In this study, a species-specific DNA fragment for Phytophthora infestans was identified and used to develop PCR and RAA-LFD assays for the detection of potato late blight pathogen. Both assays effectively distinguished P. infestans from related species and exhibited improved detection limits.

CROP PROTECTION (2023)

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Article Biochemistry & Molecular Biology

Development and application of a recombinase-aided amplification and lateral flow assay for rapid detection of pseudorabies virus from clinical crude samples

Zhenhua Guo et al.

Summary: In this study, a visible and low equipment-dependent recombinase-aid amplification assay integrated with lateral flow assay (RAA-LFA) was developed to detect the PRV gE gene. The method showed good specificity and a limit of detection of 21 copies per reaction. Clinical detection results were consistent with qPCR results. The RAA-LFA provides an accessible, accurate, and sensitive tool for point-of-care testing (POCT) of PRV in resource-limited areas.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023)

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Article Biochemistry & Molecular Biology

Rapid and Sensitive Detection of Streptococcus iniae in Trachinotus ovatus Based on Multienzyme Isothermal Rapid Amplification

Yifen Wang et al.

Summary: Establishing fast, sensitive, and convenient detection methods for S. iniae is of great significance for the early diagnosis and control of the disease in the global aquaculture industry and for humans dealing with raw fish. In this study, two methods of real-time MIRA and MIRA-LFD were established for the simA gene of S. iniae, which could complete amplification at a constant temperature of 42°C within 20 min. Real-time MIRA and MIRA-LFD assays showed high sensitivity, specificity, repeatability, simplicity, and rapidity for detecting S. iniae.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

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Article Zoology

Rapid and effective detection of Macrobrachium rosenbergii nodavirus using a combination of nucleic acid sequence-based amplification test and immunochromatographic strip

Feng Lin et al.

Summary: Nucleic acid sequence-based amplification (NASBA) combined with an immunoassay-based lateral flow dipstick (LFD) is a rapid, accurate, sensitive, and specific detection method for MrNV from China. The assay showed a sensitivity 104 times higher than the present RT-PCR approach, and it was specific for MrNV without creating products in shrimps with other virus infections. Early detection of this infectious disease is important for implementing efficient therapeutic strategies, preventing its spread, and limiting loss of aquatic breeds.

JOURNAL OF INVERTEBRATE PATHOLOGY (2023)

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Review Biochemistry & Molecular Biology

A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12/Cas13/Cas14 Proteins Utilized for Genome Engineering

V. Edwin Hillary et al.

Summary: The CRISPR/Cas system has revolutionized life science research by providing powerful tools for manipulating, detecting, imaging, and annotating DNA or RNA sequences. This review focuses on the two classes of the CRISPR/Cas system, as well as the mechanisms and applications of Cas proteins, including Cas12, Cas13, and Cas14. The review also discusses the pros and cons of various Cas proteins and their recent applications, such as detecting SARS-CoV-2.

MOLECULAR BIOTECHNOLOGY (2023)

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Article Biotechnology & Applied Microbiology

Effect of food matrix on rapid detection of Vibrio parahaemolyticus in aquatic products based on toxR gene

Darong Li et al.

Summary: Vibrio parahaemolyticus has become a significant threat to human health, and the development of rapid and accurate diagnostics is crucial for monitoring its outbreak and spread. In this study, we developed a novel assay, RAA-LFD, which combines recombinase aided amplification (RAA) with lateral flow dipstick (LFD) for the detection of V. parahaemolyticus. RAA-LFD showed excellent specificity and could detect very low levels of V. parahaemolyticus in genomic DNA and spiked food samples. However, the sensitivity of the assay was affected by the presence of food matrix, reducing the detection limit by 10 to 100 times. In field samples, RAA-LFD showed good agreement with the GB4789.7-2013 method and PCR method. Overall, RAA-LFD is a highly accurate and sensitive tool for point-of-care diagnosis of V. parahaemolyticus, meeting the increasing demand for rapid diagnostics.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2023)

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Article Biotechnology & Applied Microbiology

Simple and feasible detection of hepatitis a virus using reverse transcription multienzyme isothermal rapid amplification and lateral flow dipsticks without standard PCR laboratory

Mao-ling Sun et al.

Summary: A feasible HAV detection solution was established by combining RT-MIRA and LFD. The RT-MIRA-LFD method showed high accuracy and rapidness in detecting HAV infection. This method could provide a considerable advantage for diagnosing and controlling HAV infection, especially in regions with limited medical resources.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY (2023)

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Article Plant Sciences

Portable rapid detection of maize chlorotic mottle virus using RT-RAA/CRISPR-Cas12a based lateral flow assay

Rong Lei et al.

Summary: To effectively control maize lethal necrosis, a rapid, sensitive, and specific detection method is vital for early diagnosis of MCMV in host plant tissues. In this study, a rapid detection procedure was established by combining one-step RT-RAA and CRISPR/Cas12a-based lateral flow assay, which can be implemented on a portable metal incubator at 37-42 degrees C. The crude extract of total RNA from plant materials using alkaline-PEG buffer can be directly used as the template for one-step RT-RAA.

FRONTIERS IN PLANT SCIENCE (2023)

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Article Chemistry, Multidisciplinary

Portable Paper-Based Nucleic Acid Enrichment for Field Testing

Junyang Mei et al.

Summary: Point-of-care testing (POCT) is a preferred method for detecting infectious pathogens, including COVID-19 and certain cancers. However, the application of POCT is limited by issues like unintegrated nucleic acid extraction, open detection systems that increase the risk of cross-contamination, and dependence on expensive equipment and AC power supply. This paper reports a portable platform based on recombinase polymerase amplification (RPA) that can rapidly detect SARS-CoV-2 virus and H. pylori bacteria within 30 minutes, with a low limit of detection. The platform uses a battery-powered portable reader for on-chip amplification and fluorescence detection, and can test for multiple pathogens simultaneously.

ADVANCED SCIENCE (2023)

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Article Biophysics

Integration of on-chip lysis and paper-based sensor for rapid detection of viral and exosomal RNAs

Jingjing Qian et al.

Summary: In recent years, paper-based nucleic acid sensors have been used to detect DNA and RNA molecules extracted from viruses and bacteria. These sensors require the release of nucleic acids from lipid membranes and the removal of interfering molecules for accurate analysis. A portable sensor system has been developed to combine thermolysis and paper-based nucleic acid amplification tests (NAATs) for the detection of viral and exosomal RNA molecules. The system achieved quantitative analysis of influenza A virus (IAV) RNA and exosome microRNA within 1 hour, making it a promising point-of-care test for viral and bacterial infections.

BIOSENSORS & BIOELECTRONICS (2023)

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Article Veterinary Sciences

Integrating filter paper extraction, isothermal amplification, and lateral flow dipstick methods to detect Streptococcus agalactiae in milk within 15 min

Lingling Zhu et al.

Summary: A rapid detection method for Streptococcus agalactiae, the main pathogenic bacterium causing mastitis in dairy farming, was established. The method combines filter paper extraction, multienzyme isothermal rapid amplification, and lateral flow dipsticks. The method does not require laboratory equipment and is suitable for on-site testing.

FRONTIERS IN VETERINARY SCIENCE (2023)

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Review Chemistry, Analytical

Paper-Based Loop Mediated Isothermal Amplification (LAMP) Platforms: Integrating the Versatility of Paper Microfluidics with Accuracy of Nucleic Acid Amplification Tests

Debayan Das et al.

Summary: Paper-based diagnostics offer a cost-effective and portable alternative to traditional diagnostic methods. However, current paper-based serology tests lack the accuracy and sensitivity of molecular diagnostic tests. Loop-mediated isothermal amplification (LAMP) provides PCR-like performance while simplifying the assay complexity. This review discusses the integration of paper microfluidics with LAMP for improved accuracy and versatility in point-of-care testing.

CHEMOSENSORS (2023)

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Article Chemistry, Analytical

Monitoring Pathogenic Viable E. coli O157:H7 in Food Matrices Based on the Detection of RNA Using Isothermal Amplification and a Paper-Based Platform

Sabrina Petrucci et al.

Summary: This study developed a rapid and simple test for detecting viable E. coli O157:H7 cells by detecting the RNA of the pathogen. It is sensitive, able to distinguish between viable and nonviable bacteria, and can detect viable but nonculturable bacteria, providing timely identification and removal of contaminated consumables prior to distribution without extensive sample preparation.

ANALYTICAL CHEMISTRY (2022)

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Article Biochemistry & Molecular Biology

Combining recombinase polymerase amplification and DNA-templated reaction for SARS-CoV-2 sensing with dual fluorescence and lateral flow assay output

Lluc Farrera-Soler et al.

Summary: The early phase of the SARS-CoV-2 pandemic posed a significant diagnostic challenge. In this study, a method combining recombinase polymerase amplification and a DNA-templated reaction was developed for dual readout detection of the virus, using either fluorescence or naked eye detection. This method offers a potential solution for rapid and accurate detection of SARS-CoV-2.

BIOPOLYMERS (2022)

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Article Biophysics

Fluorescent paper-based analytical devices for ultra-sensitive dual-type RNA detections and accurate gastric cancer screening

Yuqian Liu et al.

Summary: The novel fluorescent paper-based analytical devices (PADs) developed in this study demonstrate high reliability and sensitivity for the detection of GC-related miRNA-21 and circRNA-HIAT1. These PADs show superior uniformity and stability, efficient RCA, specificity, and anti-interference capability, making them suitable for accurate GC screening in biological settings.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Food Science & Technology

A portable CRISPR Cas12a based lateral flow platform for sensitive detection of Staphylococcus aureus with double insurance

Jiajie Qian et al.

Summary: The article presents a portable and visible detection platform for S. aureus based on CRISPR/Cas12a which shows high sensitivity and specificity in pure culturing samples and artificially contaminated food samples. The platform combines ultra-sensitivity, specificity, portability, user-friendliness, and time-saving features, meeting the actual requirements for point-of-care testing and holding promise for further applications in detecting other foodborne pathogens.

FOOD CONTROL (2022)

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Article Biotechnology & Applied Microbiology

Recombinase-aided amplification combined with lateral flow dipstick for the rapid detection of Amphidinium carterae

Meiting Xu et al.

Summary: In this study, a rapid detection method for harmful algae was established using specific conjugation of biotin-labeled RAA products and carboxyfluorescein-labeled probe, which completed the entire detection process within 50 minutes. The method showed good performance in terms of specificity and sensitivity, enabling efficient detection of Amphinidium carterae.

JOURNAL OF APPLIED PHYCOLOGY (2022)

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Article Chemistry, Analytical

Tunable Duplex Semiquantitative Detection of Nucleic Acids with a Visual Lateral Flow Immunoassay Readout

Justin M. Rosenbohm et al.

Summary: Quantitative nucleic acid amplification testing is crucial for infectious disease management, but faces challenges in low resourced settings. The development of the semiquantitative ligation and amplification assay allows for semiquantitative detection of target DNA with a simple visual readout.

ANALYTICAL CHEMISTRY (2022)

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Article Biophysics

Fabrication of a fully integrated paper microdevice for point-of-care testing of infectious disease using Safranin O dye coupled with loop-mediated isothermal amplification

Vu Phong Dinh et al.

Summary: In this study, a paper microdevice that fully integrates DNA extraction, LAMP amplification, and colorimetric detection is introduced. The device successfully identifies SARS-CoV-2 and Enterococcus faecium, and serves as a rapid tool for disease control. The Safranin O-based detection method allows for visual identification of the pathogens within 60 minutes.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Microbiology

An isothermal amplification-coupled dipstick for the rapid detection of COVID-19

Rathina Kumar Shanmugakani et al.

Summary: This study reports a point-of-care detection technique for COVID-19 that combines isothermal amplification and dipstick technologies, providing a simple and rapid diagnostic test that can be performed in low-resource settings.

JOURNAL OF MEDICAL MICROBIOLOGY (2022)

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Article Chemistry, Analytical

Paper-based netlike rolling circle amplification (NRCA) for ultrasensitive and visual detection of SARS-CoV-2

Yuchen Song et al.

Summary: A new method for ultrasensitive and visual detection of SARS-CoV-2 viral nucleic acid is proposed in this study. The method integrates a paper device with highly efficient isothermal amplification technology, and possesses advantages of specificity, portability, visualization, and low cost.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

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Article Chemistry, Analytical

A chromogenic reaction-free distance-based paper device for facile detection of microRNA via viscosity amplification and surface hydrophobicity modulation

Lang Zhang et al.

Summary: In this study, a novel chromogenic reaction-free distance-based (CRFD) strategy was proposed for sensitive and accurate quantitation of microRNAs (miRNAs) using viscosity amplification and surface hydrophobicity modulations. The strategy successfully detected miR-221 and miR-222 in liver cell lysis and a smartphone-based auto-reading system was developed to improve accuracy and convenience.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

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Article Biochemical Research Methods

RPA/CRISPR/Cas12a-Based On-Site and Rapid Nucleic Acid Detection of Toxoplasma gondii in the Environment

Rong Lei et al.

Summary: A portable one-pot detection system for Toxoplasma gondii was developed, combining recombinase polymerase amplification (RPA) and CRISPR/Cas12a system. The system showed high selectivity and a limit of detection of 3.3 copies/μL. It has the advantages of rapidness, robustness, and on-site features, making it a promising tool for field applications in remote areas.

ACS SYNTHETIC BIOLOGY (2022)

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Article Engineering, Biomedical

Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples

Margot Karlikow et al.

Summary: Paper-based diagnostic tests combined with a portable device perform well for the diagnosis of Zika and chikungunya viruses in low-resource settings, as shown by double-blinded studies.

NATURE BIOMEDICAL ENGINEERING (2022)

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Article Chemistry, Analytical

A lateral flow strip biosensor platform based on cascade nucleic acid amplification technology for ultrasensitive detection of OSCC-associated salivary MicroRNA

Wenna Li et al.

Summary: Oral squamous cell carcinoma (OSCC) is a well-known malignancy that poses a serious threat to human health. This study developed a biosensor platform based on the cascade nucleic acid amplification technology for colorimetric analysis of miRNA 31, which is associated with OSCC. The platform demonstrated high sensitivity and specificity for miRNA 31 detection.

ANALYTICA CHIMICA ACTA (2022)

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Article Biochemical Research Methods

Sensitive naked-eye detection of telomerase activity based on exponential amplification reaction and lateral flow assay

Xue-Ru Cheng et al.

Summary: A LFR-EXPAR assay was developed for sensitive and easy detection of telomerase activity, combining EXPAR and lateral flow assay. This method showed comparable sensitivity to TRAP assay and has great potential for early cancer diagnosis without the need for specialized instruments.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022)

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Article Biophysics

SERS-based CRISPR/Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods

Jianwen Zhuang et al.

Summary: In this study, a RPA-Cas12a-mu PAD was designed using CRISPR/Cas12a and SERS technology for highly sensitive bacterial detection. The concentration of S.typhi was determined by the aggregation level of SERS nanoprobes, enabling fast and accurate testing of food samples.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Food Science & Technology

Visual detection of Shigella in milk by competitive annealing-mediated isothermal amplification with paper-based DNA extraction method

Xu Chen et al.

Summary: A visual CAMP assay based on the ipaH gene was developed for rapid detection of Shigella in milk, and a simple paper-based DNA extraction method was established. The combination of these two methods showed higher sensitivity and specificity for on-site detection of Shigella in milk samples.

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2022)

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Article Chemistry, Analytical

Fluorescent microspheres lateral flow assay integrated with Smartphone-based reader for multiple microRNAs detection

Fanglan He et al.

Summary: This study developed a portable and multiple miRNAs detection method, combining rolling circle amplification with fluorescent microspheres based lateral flow assay. A smartphone-based strip reader with a WeChat mini program was used to analyze the fluorescence intensity of test lines and control lines on strips for point-of-care testing.

MICROCHEMICAL JOURNAL (2022)

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Article Chemistry, Analytical

Fluorescent on-site detection of multiple pathogens using smartphone-based portable device with paper-based isothermal amplification chip

Qiaoqiao Zhou et al.

Summary: This study developed a cost-effective, portable, and easy-to-use on-site genetic diagnostics sensing system for pathogen screening. The system consists of a paper-based biochip and a smartphone-based portable device, which together enable simultaneous detection of multiple pathogens and have demonstrated practicality in foodborne pathogen detection.

MICROCHIMICA ACTA (2022)

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Article Agriculture, Dairy & Animal Science

Rapid and visual detection of Mycoplasma synoviae by recombinase-aided amplification assay combined with a lateral flow dipstick

Wenlong Xia et al.

Summary: This study established a simple, rapid, and visual detection method for Mycoplasma synoviae (MS) using recombinase-aided amplification (RAA) combined with a lateral flow dipstick (LFD). The RAA-LFD assay exhibited high sensitivity and specificity, and was suitable for the rapid detection of MS in clinical practice.

POULTRY SCIENCE (2022)

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Article Chemistry, Analytical

Sensitive lateral flow assay for bisulfite-free DNA methylation detection based on the restriction endonuclease GlaI and rolling circle amplification

Ningning Dong et al.

Summary: The detection of DNA methylation with high sensitivity and specificity is crucial for early diagnosis of human diseases. This article presents a novel DNA methylation detection method that combines the high specificity of GlaI digestion and the high amplification efficiency of rolling circle amplification (RCA), enabling rapid visual detection.

ANALYTICA CHIMICA ACTA (2022)

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Article Biochemical Research Methods

A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

Ha Neul Lee et al.

Summary: In this study, we developed a modified isothermal nucleic acid amplification method integrated with lateral flow assay (LFA) for rapid and simple genetic testing. The method involved the design of padlock probes for target DNA ligation, followed by rolling circle amplification (RCA) using capture ligand-modified oligonucleotides as primers. The amplified target DNA was then detected using gold nanoparticles on an LFA membrane strip. We successfully validated the method for detecting the mecA gene, which is associated with methicillin-resistant Staphylococcus aureus (MRSA). We also demonstrated the feasibility of using the method for viral gene targets, such as the RNA-dependent RNA polymerase (RdRp) and receptor binding domain (RBD) genes of SARS-CoV-2.

BIOCHIP JOURNAL (2022)

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Article Engineering, Environmental

Paper Device Combining CRISPR/Cas12a and Reverse-Transcription Loop-Mediated Isothermal Amplification for SARS-CoV-2 Detection in Wastewater

Haorui Cao et al.

Summary: A portable paper device based on CRISPR/Cas12a and RT-LAMP was developed for the detection of SARS-CoV-2 in wastewater, offering high sensitivity and specificity. The device enables simultaneous detection of the N, E, and S genes through different visualization pathways, and provides semi-quantitative analysis. This study demonstrates a promising point-of-use method for wastewater-based surveillance.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022)

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Article Biochemistry & Molecular Biology

Prokaryotic Argonaute Proteins as a Tool for Biotechnology

E. Kropocheva et al.

Summary: Programmable nucleases are crucial tools for manipulating genes and genomes. This review discusses the advantages and disadvantages of different genetic editors and focuses on programmable nucleases from the prokaryotic Argonaute protein family.

MOLECULAR BIOLOGY (2022)

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Article Multidisciplinary Sciences

Distance-based paper device using combined SYBR safe and gold nanoparticle probe LAMP assay to detect Leishmania among patients with HIV

Toon Ruang-areerate et al.

Summary: This study developed a highly sensitive and selective detection technique using colorimetric precipitates and fluorescence for diagnosing asymptomatic Leishmania/HIV co-infection. The method is fast, simple, inexpensive and practical, making it suitable for field diagnostics in resource-limited areas.

SCIENTIFIC REPORTS (2022)

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Article Multidisciplinary Sciences

Near-digital amplification in paper improves sensitivity and speed in biplexed reactions

Kamal G. Shah et al.

Summary: This study demonstrates how near-digital nucleic acid amplification tests can be implemented in low-cost porous media to approach the low limits of detection of digital assays, capturing many benefits of digital NAATs when performed in a paper membrane.

SCIENTIFIC REPORTS (2022)

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Article Immunology

Development and evaluation of a rapid and sensitive multienzyme isothermal rapid amplification with a lateral flow dipstick assay for detection of Acinetobacter baumannii in spiked blood specimens

Wei-Wei Hu et al.

Summary: This study established a multienzyme isothermal rapid amplification with a lateral flow dipstick (MIRA-LFD) assay for detection of A. baumannii in spiked blood specimens. The developed MIRA-LFD assay showed high specificity and sensitivity and outperformed the real-time PCR method. It is a time-saving and effective tool for rapid and reliable diagnosis of A. baumannii bloodstream infection in primary hospitals.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2022)

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Review Cell Biology

Applications of CRISPR/Cas13-Based RNA Editing in Plants

Naga Rajitha Kavuri et al.

Summary: CRISPR/Cas13-based RNA editing technologies have great potential for a wide range of applications in plants, enabling efficient manipulation of RNA without permanent changes to the genome.

CELLS (2022)

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Article Biochemistry & Molecular Biology

Detection of SARS-CoV-2 Using Reverse Transcription Helicase Dependent Amplification and Reverse Transcription Loop-Mediated Amplification Combined with Lateral Flow Assay

Aleksandra Anna Zasada et al.

Summary: This study presents the application of two nucleic acid isothermal amplification methods combined with lateral flow assay for the rapid detection of SARS-CoV-2. The RT-HDA combined with LFA method is found to be more sensitive and practical.

BIOMEDICINES (2022)

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Article Biochemical Research Methods

A microfluidic fully paper-based analytical device integrated with loop-mediated isothermal amplification and nano-biosensors for rapid, sensitive, and specific quantitative detection of infectious diseases

Hamed Tavakoli et al.

Summary: In this research, a microfluidic fully paper-based analytical device (mu FPAD) integrated with loop-mediated isothermal amplification (LAMP) and ssDNA-functionalized graphene oxide (GO) nano-biosensors was developed for the first time for simple, rapid, low-cost, and quantitative detection of Neisseria meningitidis (N. meningitidis). The presented mu FPAD offers versatile functions and is capable of a simple, highly sensitive, and specific diagnosis of N. meningitidis. Furthermore, this microfluidic approach has great potential in the rapid detection of a wide variety of different other pathogens in low-resource settings.

LAB ON A CHIP (2022)

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Article Biochemical Research Methods

Quantitative isothermal amplification on paper membranes using amplification nucleation site analysis

Benjamin P. Sullivan et al.

Summary: Quantitative nucleic acid amplification tests (qNAATs) are critical in treating infectious diseases, and this study demonstrates that recombinase polymerase amplification (RPA) reactions on paper membranes can be used to quantify HIV-1 DNA and viral RNA in less than 20 minutes. A mobile phone-based system for image capture and onboard processing is also demonstrated.

LAB ON A CHIP (2022)

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Article Chemistry, Analytical

Universal point-of-care detection of proteins based on proximity hybridization-mediated isothermal exponential amplification

Zibin Tang et al.

Summary: We have developed a point-of-care lateral flow biosensor for universal protein detection with high sensitivity and specificity using proximity hybridization-mediated protein-to-DNA signal transducer, isothermal exponential amplification, and catalytic hairpin assembly. This method provides a universal and simple approach for protein detection with a wide dynamic range and rapid detection speed.

ANALYST (2022)

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Review Chemistry, Analytical

Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments

Kuankuan Zhang et al.

Summary: This paper introduces the principles of rolling circle amplification (RCA) technology and its application in environmental analysis. It discusses the advantages and disadvantages of this method in contaminant monitoring, as well as its potential for detecting heavy metals, organic small molecules, nucleic acids, proteins, and other targets in aqueous environments.

BIOSENSORS-BASEL (2021)

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Article Biology

Recombinase-Aided Amplification Coupled with Lateral Flow Dipstick for Efficient and Accurate Detection of Porcine Parvovirus

Yihong He et al.

Summary: Porcine parvovirus (PPV) infection is a major concern in the swine industry globally, and it is crucial to establish a rapid and efficient detection method. A new recombinase-aided amplification (RAA) assay was developed in this study, which showed high specificity for PPV detection, increased sensitivity compared to PCR, and cost-saving benefits.

LIFE-BASEL (2021)

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Article Chemistry, Analytical

Microfluidic Ruler-Readout and CRISPR Cas12a-Responded Hydrogel-Integrated Paper-Based Analytical Devices (μReaCH-PAD) for Visible Quantitative Point-of-Care Testing of Invasive Fungi

Di Huang et al.

Summary: The report introduces a microfluidic ruler-readout analytical device (mu ReaCH-PAD) for visible and quantitative detection of invasive fungi (IF) using genus-conserved fragments of 18s rRNA as the detection target. The device utilizes a CRISPR Cas12a system for target recognition and a DNA hydrogel coupled with enzymatic reactions for signal amplification and transduction, allowing for easy visual quantitative readout by unaided eyes. The mu ReaCH-PAD demonstrates high sensitivity, quantitative readout, and potential as a portable detection tool of IF for early diagnosis in resource-limited settings.

ANALYTICAL CHEMISTRY (2021)

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Article Multidisciplinary Sciences

Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification

Pierre Garneret et al.

Summary: This study introduces a portable device called COVIDISC for on-site COVID-19 testing, utilizing progress in isothermal amplification and paper microfluidics. The device extracts and amplifies SARS-CoV-2 RNA isothermally using RT-LAMP technology, with detection taking between 20 minutes to 1 hour, and showing no cross-reaction with eight major respiratory viruses currently circulating in Europe. The low cost and simplicity of the device are expected to facilitate widespread dissemination and help manage pandemics.

PLOS ONE (2021)

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Article Chemistry, Multidisciplinary

Fluorometric Paper-Based, Loop-Mediated Isothermal Amplification Devices for Quantitative Point-of-Care Detection of Methicillin-Resistant Staphylococcus aureus (MRSA)

Ilada Choopara et al.

Summary: The research team developed a fluorometric paper-based LAMP technology for rapid MRSA detection. By optimizing reaction conditions, they created a paper-based MRSA diagnostic device that can accurately detect low levels of MRSA in a short amount of time, demonstrating high specificity and sensitivity in both patient and laboratory samples.

ACS SENSORS (2021)

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Article Chemistry, Analytical

Paper-based loop-mediated isothermal amplification and lateral flow (LAMP-LF) assay for identification of tissues of cattle origin

Jyoti Jawla et al.

Summary: The study developed a rapid detection method for identifying cattle tissue with high specificity and analytical sensitivity. Laboratory validation and comparison with PCR detection methods demonstrated the accuracy of the developed assay. Additionally, the assay was shown to provide rapid results in field applications.

ANALYTICA CHIMICA ACTA (2021)

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Article Biophysics

Preparation of DNA aptamer and development of lateral flow aptasensor combining recombinase polymerase amplification for detection of erythromycin

Yepeng Du et al.

Summary: The study developed ten aptamer candidates against erythromycin through the Capture-SELEX process, selecting Ery_06 with the highest enrichment for further study. The characterized aptamer showed high affinity and specificity towards erythromycin, and was used to construct a novel lateral flow aptasensor for detecting erythromycin spiked in tap water samples.

BIOSENSORS & BIOELECTRONICS (2021)

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Review Biochemistry & Molecular Biology

Rolling Circle Amplification as a Universal Method for the Analysis of a Wide Range of Biological Targets

R. R. Garafutdinov et al.

Summary: This review highlights the importance of rolling circle amplification (RCA) in the detection and analysis of biotargets, presenting key molecular participants, methods to increase efficiency and productivity, and characteristics of different reporter systems. It also demonstrates the differences in techniques for RCA-mediated analysis of biotargets of different types, along with examples of using various RCA variants to solve specific diagnostic problems.

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY (2021)

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Article Immunology

Reverse Transcription Recombinase-Aided Amplification Assay With Lateral Flow Dipstick Assay for Rapid Detection of 2019 Novel Coronavirus

Yu-Zhong Zheng et al.

Summary: This study developed a rapid and specific Point of Care Test (POCT) for detecting SARS-CoV-2 outside of the laboratory setting. The assay showed 100% specificity and sensitivity in clinical sample detection, making it a convenient tool for screening and monitoring suspected cases of COVID-19 in endemic areas.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

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Article Multidisciplinary Sciences

INSIGHT: A population-scale COVID-19 testing strategy combining point-of-care diagnosis with centralized high-throughput sequencing

Qianxin Wu et al.

Summary: INSIGHT is a two-stage COVID-19 testing strategy that combines point-of-care diagnosis with next-generation sequencing to achieve population-scale testing; the first stage provides quick decentralized readout, aiming for early isolation of asymptomatic patients and gives results within 1 to 2 hours; the second stage confirms near-patient testing results and facilitates centralized data collection.

SCIENCE ADVANCES (2021)

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Article Immunology

Rapid Detection of SARS-CoV-2 Using Duplex Reverse Transcription-Multienzyme Isothermal Rapid Amplification in a Point-of-Care Testing

Hui Chen et al.

Summary: The study developed a MIRA amplification method for rapid detection of SARS-CoV-2, showing efficient amplification of duplex targets in a low volume and monitoring with lateral flow dipsticks. The assay was able to amplify efficiently without RNA extraction and demonstrated good performance in experiments.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

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Review Biotechnology & Applied Microbiology

Paper-Based Point-of-Care Testing of SARS-CoV-2

Yuan Jia et al.

Summary: Paper-based point-of-care tests have gained interest for their rapid, low-cost, and instrument-free detection capabilities. This review focuses on the development of paper-based POC devices for detecting SARS-CoV-2, discussing principles, performance, advantages, drawbacks, limitations, and prospects of existing devices in the field.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2021)

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Article Biochemistry & Molecular Biology

Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip

Mao-Ling Sun et al.

Summary: A new method for detecting HBV using a combination of MIRA and LFD technologies has been developed, which is simple, rapid, and accurate, making it particularly suitable for resource-limited regions.

FRONTIERS IN MOLECULAR BIOSCIENCES (2021)

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Article Parasitology

Recombinase polymerase amplification with lateral flow strip for detecting Babesia microti infections

Zheng Nie et al.

Summary: The LF-RPA assay targeting B. microti is a new, simple, specific, sensitive, rapid, and convenient method for diagnosing infection with Babesia. Compared to conventional PCR, it has higher sensitivity and faster detection capabilities, making it suitable for field applications and large-scale sample screening.

PARASITOLOGY INTERNATIONAL (2021)

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Article Chemistry, Analytical

A simple and low-cost paper-based colorimetric method for detecting and distinguishing the GII.4 and GII.17 genotypes of norovirus

Qiuli Sun et al.

Summary: Norovirus detection remains a challenge, but a simple and rapid detection method has been established based on a paper-based colorimetric system, showing high specificity in differentiating various viruses and suggesting potential as a low-cost visual detection method for pathogens in clinical samples, especially in remote areas.

TALANTA (2021)

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Article Multidisciplinary Sciences

A simple and rapid method for fish sex identification based on recombinase-aided amplification and its use in Cynoglossus semilaevis

Zhichao Nie et al.

Summary: The study established a recombinase-aided amplification (RAA)-based method for fish sex identification using Cynoglossus semilaevis as an example, which combines two result visualization approaches with distinct features - capillary electrophoresis (CE) and lateral flow dipstick (LFD). The RAA-LFD method is operationally simple, rapid (around 30 minutes), and holds great potential for point-of-need applications in fisheries and other fields.

SCIENTIFIC REPORTS (2021)

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Article Fisheries

A lateral flow biosensor for rapid detection of Singapore grouper iridovirus (SGIV)

Jiaxin Liu et al.

Summary: Iridoviruses are known to cause serious diseases in fish species worldwide. Singapore grouper iridovirus (SGIV) has caused significant economic losses in grouper aquaculture without effective therapies. A novel LFB combined with DNA aptamers has been developed for rapid and sensitive detection of SGIV infection, showing high specificity and sensitivity in vitro and in vivo.

AQUACULTURE (2021)

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Article Biophysics

Detection and differentiation of respiratory syncytial virus subgroups A and B with colorimetric toehold switch sensors in a paper-based cell-free system

Mengcen Cao et al.

Summary: The method utilizes colorimetric toehold switch sensors in a paper-based cell-free system for sensitive detection and differentiation of RSV subgroups A and B, with improved sensitivity using nucleic acid sequence-based amplification. It can generate visible results without specialized operators or sophisticated equipment, making it suitable for use in resource-poor areas and point-of-care testing scenarios.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Microbiology

A newly developed paper embedded microchip based on LAMP for rapid multiple detections of foodborne pathogens

Mimi Zhang et al.

Summary: The paper embedded LAMP microchip provides an inexpensive, user-friendly, and highly selective pathogen detection method, showing great potential for quick, efficient, and cost-effective foodborne pathogen detection in the future.

BMC MICROBIOLOGY (2021)

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Article Chemistry, Applied

On-site paper-based Loop-Mediated Isothermal Amplification coupled Lateral Flow Assay for pig tissue identification targeting mitochondrial CO I gene

Jyoti Jawla et al.

Summary: The study developed a LAMP method for detecting tissues of pig origin and designed a paper-based LAMP-LFA kit for quick and efficient identification of tissue of pig origin.

JOURNAL OF FOOD COMPOSITION AND ANALYSIS (2021)

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Article Chemistry, Analytical

LAMP-CRISPR-Cas12-based diagnostic platform for detection of Mycobacterium tuberculosis complex using real-time fluorescence or lateral flow test

Yi Wang et al.

Summary: The LACD system utilizes engineered core primers with PAM sites to enable CRISPR-Cas12a mediated detection in the LAMP stage.

MICROCHIMICA ACTA (2021)

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Article Chemistry, Multidisciplinary

Dual Methylation-Sensitive Restriction Endonucleases Coupling with an RPA-Assisted CRISPR/Cas13a System (DESCS) for Highly Sensitive Analysis of DNA Methylation and Its Application for Point-of-Care Detection

Xianfeng Wang et al.

Summary: The DESC system utilizes a digestion strategy based on dual methylation-sensitive restriction endonucleases and RPA-assisted CRISPR/Cas13a system for accurate and sensitive determination of site-specific DNA methylation. This system allows rapid signal amplification and effectively eliminates false positive influences from incomplete digestion of unmethylated targets. With high sensitivity, outstanding selectivity, and ease of operation, the DESCS system provides a reliable method for site-specific analysis of methylation.

ACS SENSORS (2021)

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Review Cell Biology

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

Marianna Soroka et al.

Summary: LAMP is a rapid amplification method that can produce up to 10(9) copies of amplified DNA within less than an hour, with high specificity and stable temperature operation. It can detect products faster than standard techniques and has the potential to be a rapid diagnostic tool for SARS-CoV-2.

CELLS (2021)

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Proceedings Paper Engineering, Electrical & Electronic

Rapid MicroRNA Detection Using Paper-Based Isothermal Amplification

Jingjing Qian et al.

Summary: This study presents a paper-based miRNA sensor system that can detect miRNA-223 with a concentration as low as 10 pM within one hour.

2021 IEEE SENSORS (2021)

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Article Chemistry, Analytical

Visual assay of Escherichia coli O157:H7 based on an isothermal strand displacement and hybrid chain reaction amplification strategy

Xiu Liu et al.

Summary: A simple, sensitive, and enzyme-free method for visual point-of-care detection of 16S rRNA of Escherichia coli O157:H7 has been developed based on an isothermal strand displacement-hybrid chain reaction (ISD-HCR) and lateral flow strip (LFS). This approach allows for detection of E. coli O157:H7 at concentrations as low as 10(2) CFU mL(-1) without the need for instrumentation, providing a valuable tool for point-of-care detection of pathogenic bacteria in resource-limited countries.

ANALYTICAL METHODS (2021)

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Article Chemistry, Analytical

Padlock probe-based rolling circle amplification lateral flow assay for point-of-need nucleic acid detection

Sidhartha Jain et al.

Summary: The sensitive nucleic acid detection method based on padlock probe rolling circle amplification, nuclease protection, and lateral flow detection has been developed for use in resource-limited settings. By combining these techniques, the method allows for detection of antimicrobial resistance genes and specific pathogens such as SARS-CoV-2 with high sensitivity.

ANALYST (2021)

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Article Agriculture, Dairy & Animal Science

Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of avian infectious bronchitis virus

Wenjing Wang et al.

POULTRY SCIENCE (2020)

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Article Chemistry, Analytical

A highly integrated system with rapid DNA extraction, recombinase polymerase amplification, and lateral flow biosensor for on-site detection of genetically modified crops

Xiaofu Wang et al.

ANALYTICA CHIMICA ACTA (2020)

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Article Biotechnology & Applied Microbiology

Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

Robert Player et al.

BMC GENOMICS (2020)

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Article Infectious Diseases

Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection

Guanhua Xue et al.

BMC INFECTIOUS DISEASES (2020)

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Article Chemistry, Multidisciplinary

Protein-Mediated Suppression of Rolling Circle Amplification for Biosensing with an Aptamer-Containing DNA Primer

Roger M. Bialy et al.

CHEMISTRY-A EUROPEAN JOURNAL (2020)

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Article Agronomy

Evaluation of CRISPR/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in rice

Yun-mu Zhang et al.

MOLECULAR BREEDING (2020)

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Article Chemistry, Multidisciplinary

Polymerization-Based Amplification for Target-Specific Colorimetric Detection of Amplified Mycobacterium tuberculosis DNA on Cellulose

Emma H. Yee et al.

ACS SENSORS (2020)

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Review Biophysics

Nucleic acid amplification free biosensors for pathogen detection

Yanju Chen et al.

BIOSENSORS & BIOELECTRONICS (2020)

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Article Veterinary Sciences

Rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick

Xiang Gao et al.

BMC VETERINARY RESEARCH (2020)

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Article Chemistry, Multidisciplinary

CRISPR/Cas13a Powered Portable Electrochemiluminescence Chip for Ultrasensitive and Specific MiRNA Detection

Ting Zhou et al.

ADVANCED SCIENCE (2020)

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Article Biochemical Research Methods

Method for the elucidation of LAMP products captured on lateral flow strips in a point of care test for HPV 16

Lena Landaverde et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2020)

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Article Environmental Sciences

Detection ofProrocentrum minimumby hyperbranched rolling circle amplification coupled with lateral flow dipstick

Fuguo Liu et al.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020)

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Article Marine & Freshwater Biology

Establishment of double probes rolling circle amplification combined with lateral flow dipstick for rapid detection of Chattonella marina

Yue Qin et al.

HARMFUL ALGAE (2020)

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Article Biophysics

Sub-attomole detection of HIV-1 using padlock probes and rolling circle amplification combined with microfluidic affinity chromatography

Ruben R. G. Soares et al.

BIOSENSORS & BIOELECTRONICS (2020)

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Article Biochemical Research Methods

Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks

Michael G. Mason et al.

NATURE PROTOCOLS (2020)

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Article Biophysics

An IoT-enabled paper sensor platform for real-time analysis of isothermal nucleic acid amplification tests

Mingdian Liu et al.

BIOSENSORS & BIOELECTRONICS (2020)

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Article Multidisciplinary Sciences

Development of a reverse transcription recombinase polymerase based isothermal amplification coupled with lateral flow immunochromatographic assay (CTV-RT-RPA-LFICA) for rapid detection of Citrus tristeza virus

Dilip Kumar Ghosh et al.

SCIENTIFIC REPORTS (2020)

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Article Chemistry, Analytical

Development of a fluorescent distance-based paper device using loop-mediated isothermal amplification to detect Escherichia coli in urine

Natkrittaya Saengsawang et al.

ANALYST (2020)

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Article Chemistry, Analytical

A lateral flow biosensor based on gold nanoparticles detects four hemorrhagic fever viruses

Jin Zhao et al.

ANALYTICAL METHODS (2020)

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Article Biotechnology & Applied Microbiology

Combinations of PCR and Isothermal Amplification Techniques Are Suitable for Fast and Sensitive Detection of SARS-CoV-2 Viral RNA

Dmitriy A. Varlamov et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

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Review Immunology

Detecting amplicons of loop-mediated isothermal amplification

Kazuya Shirato

MICROBIOLOGY AND IMMUNOLOGY (2019)

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A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Jaime A. Cardona-Ospina et al.

PREVENTIVE VETERINARY MEDICINE (2019)

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An Origami Paper-Based Device Printed with DNAzyme-Containing DNA Superstructures for Escherichia coli Detection

Yating Sun et al.

MICROMACHINES (2019)

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Review Chemistry, Analytical

Paper-based nucleic acid amplification tests for point-of-care diagnostics

Navjot Kaur et al.

ANALYST (2018)

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Article Chemistry, Analytical

Competitive annealing mediated isothermal amplification of nucleic acids

Rui Mao et al.

ANALYST (2018)

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Review Chemistry, Multidisciplinary

Exponential Isothermal Amplification of Nucleic Acids and Assays for Proteins, Cells, Small Molecules, and Enzyme Activities: An EXPAR Example

Michael S. Reid et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

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Article Medical Laboratory Technology

FTA Cards for Preservation of Nucleic Acids for Molecular Assays A Review on the Use of Cytologic/Tissue Samples

Gilda da Cunha Santos

ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE (2018)

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Article Chemistry, Multidisciplinary

Paper-Based DNA Reader for Visualized Quantification of Soil-Transmitted Helminth Infections

Alex Guan Wang et al.

ACS SENSORS (2018)

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Article Chemistry, Analytical

Single-Step Recombinase Polymerase Amplification Assay Based on a Paper Chip for Simultaneous Detection of Multiple Foodborne Pathogens

Heeseop Ahn et al.

ANALYTICAL CHEMISTRY (2018)

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Review Multidisciplinary Sciences

DNA interference and beyond: structure and functions of prokaryotic Argonaute proteins

Lidiya Lisitskaya et al.

NATURE COMMUNICATIONS (2018)

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Correction Chemistry, Multidisciplinary

The potential of paper-based diagnostics to meet the ASSURED criteria (vol 8, pg 34012, 2018)

Suzanne Smith et al.

RSC ADVANCES (2018)

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Rapid detection of Salmonella with Recombinase Aided Amplification

Xiaoping Zhang et al.

JOURNAL OF MICROBIOLOGICAL METHODS (2017)

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Review Biochemical Research Methods

Paper microfluidics for nucleic acid amplification testing (NAAT) of infectious diseases

Laura Magro et al.

LAB ON A CHIP (2017)

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Article Medicine, Research & Experimental

A Paper-Based Device for Performing Loop-Mediated Isothermal Amplification with Real-Time Simultaneous Detection of Multiple DNA Targets

Youngung Seok et al.

THERANOSTICS (2017)

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A High-Throughput Standard PCR-Based Genotyping Method for Determining Transgene Zygosity in Segregating Plant Populations

Lige Geng et al.

FRONTIERS IN PLANT SCIENCE (2017)

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Review Chemistry, Multidisciplinary

The Discovery of Rolling Circle Amplification and Rolling Circle Transcription

Michael G. Mohsen et al.

ACCOUNTS OF CHEMICAL RESEARCH (2016)

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Engineering long shelf life multilayer biologically active surfaces on microfluidic devices for point of care applications

Waseem Asghar et al.

SCIENTIFIC REPORTS (2016)

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Article Chemistry, Analytical

Paper-Based RNA Extraction, in Situ Isothermal Amplification, and Lateral Flow Detection for Low-Cost, Rapid Diagnosis of Influenza A (H1N1) from Clinical Specimens

Natalia M. Rodriguez et al.

ANALYTICAL CHEMISTRY (2015)

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Review Biophysics

Advances in isothermal amplification: novel strategies inspired by biological processes

Jia Li et al.

BIOSENSORS & BIOELECTRONICS (2015)

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Isothermal Amplification of Nucleic Acids

Yongxi Zhao et al.

CHEMICAL REVIEWS (2015)

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Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects

Tsugunori Notomi et al.

JOURNAL OF MICROBIOLOGY (2015)

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Review Chemistry, Multidisciplinary

Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine

M. Monsur Ali et al.

CHEMICAL SOCIETY REVIEWS (2014)

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Article Biochemical Research Methods

Paper-based microfluidics with an erodible polymeric bridge giving controlled release and timed flow shutoff

Sana Jahanshahi-Anbuhi et al.

LAB ON A CHIP (2014)

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Article Biochemical Research Methods

A device architecture for three-dimensional, patterned paper immunoassays

Jeremy E. Schonhorn et al.

LAB ON A CHIP (2014)

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Article Biochemical Research Methods

A perspective on paper-based microfluidics: Current status and future trends

Xu Li et al.

BIOMICROFLUIDICS (2012)

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Direct Detection of Point Mutations in Nonamplified Human Genomic DNA

Roberta D'Agata et al.

ANALYTICAL CHEMISTRY (2011)

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Review Biochemical Research Methods

Miniaturized isothermal nucleic acid amplification, a review

Peter J. Asiello et al.

LAB ON A CHIP (2011)

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Review Chemistry, Analytical

Bioactive paper provides a low-cost platform for diagnostics

Robert Pelton

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2009)

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Article Biochemical Research Methods

FLASH: A rapid method for prototyping paper-based microfluidic devices

Andres W. Martinez et al.

LAB ON A CHIP (2008)

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Article Multidisciplinary Sciences

Three-dimensional microfluidic devices fabricated in layered paper and tape

Andres W. Martinez et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)

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Article Biochemistry & Molecular Biology

DNA detection using recombination proteins

Olaf Piepenburg et al.

PLOS BIOLOGY (2006)

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Article Biochemistry & Molecular Biology

Helicase-dependent isothermal DNA amplification

M Vincent et al.

EMBO REPORTS (2004)

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Review Biotechnology & Applied Microbiology

Advances in biosensors for detection of pathogens in food and water

P Leonard et al.

ENZYME AND MICROBIAL TECHNOLOGY (2003)

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Article Biochemistry & Molecular Biology

Loop-mediated isothermal amplification of DNA

Tsugunori Notomi et al.

NUCLEIC ACIDS RESEARCH (2000)

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Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests

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