Journal
BIOSENSORS-BASEL
Volume 13, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/bios13070721
Keywords
resistive pulse sensor; signal multiplexing; bipolar pulse; high throughput; particle counting; microfluidics; iterative cancellation
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Rapid and accurate analysis of micro/nano bio-objects is crucial in clinical diagnostics and drug discovery. A unique bipolar pulse-width, multiplexing-based resistive pulse sensor is presented for high-throughput analysis of microparticles. The accuracy of particle counting and sizing was validated and its accuracy for cell analysis was demonstrated.
Rapid and accurate analysis of micro/nano bio-objects (e.g., cells, biomolecules) is crucial in clinical diagnostics and drug discovery. While a traditional resistive pulse sensor can provide multiple kinds of information (size, count, surface charge, etc.) about analytes, it has low throughput. We present a unique bipolar pulse-width, multiplexing-based resistive pulse sensor for high-throughput analysis of microparticles. Signal multiplexing is enabled by exposing the central electrode at different locations inside the parallel sensing channels. Together with two common electrodes, the central electrode encodes the electrical signal from each sensing channel, generating specific bipolar template waveforms with different pulse widths. Only one DC source is needed as input, and only one combined electrical output is collected. The combined signal can be demodulated using correlation analysis and a unique iterative cancellation scheme. The accuracy of particle counting and sizing was validated using mixtures of various sized microparticles. Results showed errors of 2.6% and 6.1% in sizing and counting, respectively. We further demonstrated its accuracy for cell analysis using HeLa cells.
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