Journal
BIOSENSORS-BASEL
Volume 13, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/bios13090876
Keywords
albuminuria; aptasensor; fluorescence quenching; portable fluorometer; chronic kidney disease; renal disease
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This study presents the development of a portable fluorometer with a smartphone application designed for the early screening of kidney diseases. The device achieved a linear calibration curve and a detection limit for human serum albumin, and demonstrated comparable performance with a commercial fluorometer and the standard automated method when analyzing 130 urine samples. These findings validate the feasibility and reliability of the portable fluorometer and aptasensor combination.
This study presents the development of a portable fluorometer with a smartphone application designed to facilitate the early screening of chronic kidney and renal diseases by enabling the sensitive detection of urinary albumin. Utilizing a fluorescence-based aptasensor, the device achieved a linear calibration curve (0.001-1.5 mg/mL) with a linearity of up to 0.98022 and a detection limit of 0.203 & mu;g/mL for human serum albumin (HSA). The analysis of 130 urine samples demonstrated comparable performance between this study's fluorometer, a commercial fluorometer, and the standard automated method. These findings validate the feasibility of the portable fluorometer and aptasensor combination as a reliable instrument for the sensitive and specific measurement of HSA in urine samples. Moreover, the fluorometer's portability offers potential applications in portable point-of-care testing, enhancing its utility in clinical settings for early disease screening.
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