The Effect of Month of Harvesting and Temperature-Humidity Index on the Number and Quality of Oocytes and In Vitro Embryo Production in Holstein Cows and Heifers

The aim of this study was to evaluate the effect of the month of oocyte harvesting and the temperature-humidity index on oocyte number and quality harvested from Holstein cows and heifers, oocyte developmental competence, and total embryos produced in an area of intense ambient temperature for most of the year. A total of 5064 multiparous lactating cows and 2988 nulliparous heifers were used as oocyte donors and distributed across the months of the year. Overall, total oocytes per collection did not differ (p > 0.05) between cows (16.6 +/- 2.7) and heifers (15.1 +/- 1.8), but oocyte developmental competence was lower (p < 0.05) in cows (21.3 +/- 5.4) than heifers (25.5 +/- 4.0). For cows, the total number of oocytes harvested was two-fold higher (p < 0.05) in November than in May. For heifers, the total number of oocytes harvested was highest in April (17.19 +/- 0.53) and lowest in May (10.94 +/- 0.32; p < 0.05). For cows, total embryos were highest in November (2.58 +/- 0.42) and lowest in August (1.28 +/- 0.10; p < 0.05). Thus, taken together, these results indicate that severe heat stress impaired the number and quality of oocytes harvested from donor Holstein multiparous cows and heifers, oocyte developmental competence, and total embryos produced in this area of intense ambient temperature for most of the year.

Automated summary

This study evaluated the impact of the month of oocyte harvesting and the temperature-humidity index on oocyte number and quality, oocyte developmental competence, and total embryos produced from Holstein cows and heifers in an area with intense ambient temperatures. Results showed that severe heat stress adversely affected the number and quality of oocytes collected, oocyte developmental competence, and total embryo production from Holstein cows and heifers in this region.