Journal
ISCIENCE
Volume 26, Issue 8, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.isci.2023.107479
Keywords
-
Categories
Ask authors/readers for more resources
The research revealed significant transcriptional similarity between FAPs and MeSCs, confirming PDGFRα as a suitable marker for FAPs and identifying extracellular proteolysis as a new function of FAPs. Additionally, decreased FAP-mediated Wnt signaling may be a potential driver of FAP dysfunction in dysferlinopathic muscles. Furthermore, analysis of FAPs in dystrophin-deficient muscles showed a relationship between muscle pathology and alteration in FAP gene expression.
Fibro/adipogenic progenitors (FAPs) are skeletal muscle stromal cells that support regeneration of injured myofibers and their maintenance in healthy muscles. FAPs are related to mesenchymal stem cells (MSCs/MeSCs) found in other adult tissues, but there is poor understanding of the extent of similarity between these cells. Using single-cell RNA sequencing (scRNA-seq) datasets from multiple mouse tissues, we have performed comparative transcriptomic analysis. This identified remarkable transcriptional similarity between FAPs and MeSCs, confirmed the suitability of PDGFR alpha as a reporter for FAPs, and identified extracellular proteolysis as a new FAP function. Using PDGFR alpha as a cell surface marker, we isolated FAPs from healthy and dysferlinopathic mouse muscles and performed scRNA-seq analysis. This revealed decreased FAP-mediated Wnt signaling as a potential driver of FAP dysfunction in dysferlinopathic muscles. Analysis of FAPs in dysferlin-and dystrophin-deficient muscles identified a relationship between the nature of muscle pathology and alteration in FAP gene expression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available