Journal
ISCIENCE
Volume 26, Issue 12, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.isci.2023.108389
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This study clarifies the function of archaeal canonical SSBs, which are not essential for cell viability but play a role in melting dsRNA or DNA/RNA hybrids.
Single-stranded DNA-binding proteins (SSBs) have been regarded as indispensable replication factors. Herein, we report that the genes encoding the canonical SSB (SisSSB) and the non-canonical SSB (SisDBP) in Saccharolobus islandicus REY15A are not essential for cell viability. Interestingly, at a lower temperature (55 degrees C), the protein level of SisSSB increases and the growth of DSisssb and DSisssbDSisdbp is retarded. SisSSB exhibits melting activity on dsRNA and DNA/RNA hybrid in vitro and is able to melt RNA hairpin in Escherichia coli. Furthermore, the core SisSSB domain is able to complement the absence of cold-shock proteins in E. coli. Importantly, these activities are conserved in the canonical SSBs from Crenarchaeota species that lack bacterial Csp homologs. Overall, our study has clarified the function of the archaeal canonical SSBs which do not function as a DNA-processing factor, but play a role in the processes requiring melting of dsRNA or DNA/RNA hybrid.
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