Journal
NATURE PROTOCOLS
Volume 11, Issue 4, Pages 747-763Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2016.040
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Funding
- Lilly Fellowship
- Chapman Foundation Fellowship
- US National Institutes of Health (NIH) [R37 DK43051, P30 DK57521, R01 DK098002]
- JPB foundation
- National Cancer Institute, Cancer Center Support Grant [P30 CA014195]
- Leona M. and Harry B. Helmsley Charitable Trust [2012-PG-MED002]
- Dr. Frederick Paulsen Chair/Ferring Pharmaceuticals
- Swedish Research Council
- Novo Nordisk Foundation
- Torsten Soderberg Foundation
- Heart-Lung Foundation
- Sahlgrenska University Hospital ALF funds
- EU Seventh Framework Program RESOLVE
- Novo Nordisk Fonden [NNF15OC0016484] Funding Source: researchfish
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Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of endogenous mammalian lipids with antidiabetic and anti-inflammatory effects. We previously identified 16 different FAHFA families, such as branched palmitic acid esters of hydroxy stearic acids (PAHSAs); each family consists of multiple isomers in which the branched ester is at different positions (e.g., 5- and 9-PAHSA). We anticipate increased need for PAHSA measurements as markers of metabolic and inflammatory health. In this protocol, we provide a detailed description of the extraction of FAHFAs from human or mouse tissues, their enrichment by solid-phase extraction and subsequent analysis by LCLC-MS. For a sample size of 6-12, the time frame is 2-3 d.
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