Seamlessly Adhesive Bionic Periosteum Patches Via Filling Microcracks for Defective Bone Healing

Current artificial designs of the periosteum focus on osteogenic or angiogenic properties, while ignoring the filling and integration with bone microcracks, which trigger a prolonged excessive inflammatory reaction and lead to failure of bone regeneration. In this study, seamless adhesive biomimetic periosteum patches (HABP/Sr-PLA) were prepared to fill microcracks in defective bone via interfacial self-assembly induced by Sr ions mediated metal-ligand interactions among pamidronate disodium-modified hyaluronic acid (HAPD), black phosphorus (BP), and hydrophilic polylactic acid (PLA). In vitro, HABP/Sr-PLA exhibited excellent self-healing properties, seamlessly filled bone microcracks, and significantly enhanced osteogenesis and angiogenesis. Furthermore, in a rat cranial defect model, HABP/Sr-PLA was demonstrated to significantly promote the formation of blood vessels and new bone under mild 808 nm photothermal stimulation (42.8 & DEG;C), and the highest protein expression of CD31 and OPN was five times higher than that of the control group and other groups. Therefore, the proposed seamless microcrack-filled bionic periosteum patch is a promising clinical strategy for promoting bone repair.

Automated summary

In this study, seamless adhesive biomimetic periosteum patches were developed to fill microcracks in defective bone and promote bone regeneration. The patches exhibited excellent self-healing properties, enhanced osteogenesis and angiogenesis, and significantly promoted blood vessel and new bone formation in a rat cranial defect model. Therefore, the proposed biomimetic periosteum patch is a promising clinical strategy for promoting bone repair.