Journal
NATURE PROTOCOLS
Volume 11, Issue 4, Pages 714-726Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2016.038
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Funding
- Center for Integration of Medicine and Innovative Technology (CIMIT)'s Point-of-Care Technology Center in Primary Care (POCTRN) Grant
- University of Illinois at Urbana-Champaign
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Enumerating specific cell types from whole blood can be very useful for research and diagnostic purposes-e.g., for counting of CD4 and CD8 T cells in HIV/AIDS diagnostics. We have developed a biosensor based on a differential immunocapture technology to enumerate specific cells in 30 min using 10 mu l of blood. This paper provides a comprehensive stepwise protocol to replicate our biosensor for CD4 and CD8 cell counts. The biochip can also be adapted to enumerate other specific cell types such as somatic cells or cells from tissue or liquid biopsies. Capture of other specific cells requires immobilization of their corresponding antibodies within the capture chamber. Therefore, this protocol is useful for research into areas surrounding immunocapture-based biosensor development. The biosensor production requires 24 h, a one-time cell capture optimization takes 6-9 h, and the final cell counting experiment in a laboratory environment requires 30 min to complete.
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