A 24-h restraint with food and water deprivation: a potential method to establish a model of depression in pigs

Adverse stress, such as the long-term restriction of food intake and activity in intensive production, leads to a depression-like mental state in sows. Mood disorder, such as depression, is a widely concerned animal welfare issue. However, little is known about the biological mechanisms that underlie mood disorders in pigs. This study is the first attempt to establish a pig depression model by acute stress. A total of 16 adult Bama pigs were divided into the control and model groups, with 8 pigs (half male and half female) per group. The pigs in the model group were restrained for 24 h in a dark and ventilated environment, with food and water deprivation. After the restraint, behavioral tests (feed intake, sucrose preference test, open field test, and novel object test) were used to evaluate apparent indicators. The levels of COR and ACTH in the serum and the levels of 5-HT, NE, and BDNF in the hippocampus and medial prefrontal cortex were detected using ELISA to identify the physiological state. After acute stress, pigs exhibited decreased feed intake and sucrose preference, increased serum COR levels, decreased hippocampal 5-HT levels, and exhibited more fear. Finally, the model was evaluated according to the weight of the test indicators. The overall score of the model was 0.57, indicating that modeling was feasible. Although the reliability and stability require further verification, this novel model revealed typical depression-like changes in behavior and provided a potential method to establish a model of depression in pigs.

Automated summary

Adverse stress, such as long-term food restriction and activity limitation, can lead to depression-like mental state in sows. This study aimed to establish a pig depression model using acute stress and evaluate the model's feasibility. Results showed that after acute stress, pigs exhibited decreased feed intake, sucrose preference, and hippocampal 5-HT levels, as well as increased serum COR levels and fear. The overall score for the model was 0.57, suggesting that modeling was feasible. This novel model provides insight into the biological mechanisms underlying mood disorders in pigs.