Journal
NATURE NANOTECHNOLOGY
Volume 12, Issue 3, Pages 274-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NNANO.2016.239
Keywords
-
Funding
- Canadian Institutes of Health Research (Emerging Team grant, POP grant)
- Ontario Research Fund (ORF Research Excellence grant)
- Canadian Cancer Society Research Institute (Innovation grant)
- Connaught Foundation
Ask authors/readers for more resources
Profiling the heterogeneous phenotypes of rare circulating tumour cells (CTCs) in whole blood is critical to unravelling the complex and dynamic properties of these potential clinical markers. This task is challenging because these cells are present at parts per billion levels among normal blood cells. Here we report a new nanoparticle-enabled method for CTC characterization, called magnetic ranking cytometry, which profiles CTCs on the basis of their surface expression phenotype. We achieve this using a microfluidic chip that successfully processes whole blood samples. The approach classifies CTCs with single-cell resolution in accordance with their expression of phenotypic surface markers, which is read out using magnetic nanoparticles. We deploy this new technique to reveal the dynamic phenotypes of CTCs in unprocessed blood from mice as a function of tumour growth and aggressiveness. We also test magnetic ranking cytometry using blood samples collected from cancer patients.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available