Journal
Nature Nanotechnology
Volume 11, Issue 8, Pages 713-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NNANO.2016.66
Keywords
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Funding
- National Natural Science Foundation of China [21327807, 21421004]
- 111 Project [B16017]
- National Basic Research Program of China (973 Program) [2013CB733700]
- Chang Jiang Scholars Program
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Protein nanopores offer an inexpensive, label-free method of analysing single oligonucleotides. The sensitivity of the approach is largely determined by the characteristics of the pore-forming protein employed, and typically relies on nanopores that have been chemically modified or incorporate molecular motors. Effective, high-resolution discrimination of oligonucleotides using wild-type biological nanopores remains difficult to achieve. Here, we show that a wild-type aerolysin nanopore can resolve individual short oligonucleotides that are 2 to 10 bases long. The sensing capabilities are attributed to the geometry of aerolysin and the electrostatic interactions between the nanopore and the oligonucleotides. We also show that the wild-type aerolysin nanopores can distinguish individual oligonucleotides from mixtures and can monitor the stepwise cleavage of oligonucleotides by exonuclease I.
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