Green method for determination of four anti-viral drugs using micellar liquid chromatography: Application to dosage form analysis

A micellar liquid chromatographic (MLC) method was developed to separate and analyze four anti-viral drugs. The method utilized a Thermo Hypersil ODS C18 HPLC column with an isocratic elution mode employing a mobile phase consisting of sodium dodecyl sulfate (0.15 M, pH 3), iso-propanol and triethylamine (79.7:20:0.3, v/v/v). The Design Expert 8 & REG; software was used to op-timize four factors simultaneously and the developed quadratic D-optimal model could achieve maximum resolution between peaks while minimizing the run time. The method was found linear over the concentration ranges of 10-100 & mu;g/mL for sofosbuvir, daclatasvir, and 20-200 & mu;g/mL for ledipasvir and velpatasvir, and successfully applied to the analysis of sofosbuvir and vel-patasvir in tablets. The MLC method was compared with a previously reported high-performance liquid chromatography (HPLC) method using the analytical GREEnness metric approach (AGREE), and was found to exhibit greener performance due to the use of safer reagents. The re-sults indicate the high potential of micellar liquid chromatography in the separation of quater-nary mixtures with the aid of response surface methodologies.

Automated summary

A micellar liquid chromatographic method was developed for the separation and analysis of four anti-viral drugs. The method utilized a Thermo Hypersil ODS C18 HPLC column with an isocratic elution mode. By optimizing four factors simultaneously using Design Expert 8 software, a quadratic D-optimal model was created to achieve maximum resolution between peaks and minimize run time. The method was found to be linear and successfully applied to the analysis of the drugs in tablets.