Journal
NATURE METHODS
Volume 13, Issue 9, Pages 751-754Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3930
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Funding
- BBSRC TRDF [BB/M020061/1]
- BBSRC [BB/M020061/1, BB/M012336/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M012336/1, BB/M020061/1] Funding Source: researchfish
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The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.
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