Journal
NATURE METHODS
Volume 14, Issue 1, Pages 53-56Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4074
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Funding
- 'Middelgroot' investment grant [834.09.003]
- CW-Echo [711.011.018, 711.013.009]
- ALW-VIDI grant from Netherlands Organization for Scientific Research (NWO) [864.09.015]
- Foundation for Technological Sciences (STW) from the Netherlands [12149]
- European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant [706443]
- Marie Curie Actions (MSCA) [706443] Funding Source: Marie Curie Actions (MSCA)
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We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Forster resonance energy transfer (FRET) acceptor in ratiometric imaging.
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