Journal
NATURE METHODS
Volume 13, Issue 12, Pages 993-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4045
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Funding
- Siebel Scholar Award
- Stanford Graduate Fellowship
- Howard Hughes Medical Institute International Student Research Fellowship
- NIH Molecular Biophysics Predoctoral Research [5T32GM008294]
- Stanford Research Experience for Undergraduates
- NIH [R01GM118377, P50GM107615]
- Burroughs Wellcome Foundation Career Award for Medical Scientists
- Rita Allen Foundation Scholar Award
- Pioneer Award [5DP1GM111003]
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A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.
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