Journal
NATURE METHODS
Volume 13, Issue 6, Pages 528-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3861
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Funding
- US National Institutes of Health (EUREKA grant) [GM085448]
- US National Institutes of Health (BIRC grant) [P41GM10369]
- US National Institutes of Health (Common Fund Glycoscience grant) [U01GM116254]
- US Department of Health and Human Services [HHSN272201400004C]
- Defense Advanced Research Projects Agency [HR0011-10-00]
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Glycans have essential roles in biology and the etiology of many diseases. A major hurdle in studying glycans through functional glycomics is the lack of methods to release glycans from diverse types of biological samples. Here we describe an oxidative strategy using household bleach to release all types of free reducing N-glycans and O-glycan-acids from glycoproteins, and glycan nitriles from glycosphingolipids. Released glycans are directly useful in glycomic analyses and can be derivatized fluorescently for functional glycomics. This chemical method overcomes the limitations in glycan generation and promotes archiving and characterization of human and animal glycomes and their functions.
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