Journal
NATURE METHODS
Volume 13, Issue 10, Pages 841-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/nmeth.3965
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Funding
- European Research Council [339778]
- CASE studentship from Biotechnology and Biological Sciences Research Council (BBSRC)
- Illumina [BB/I015477/1]
- Herchel Smith Fellowship
- Croucher Foundation
- Wellcome Trust [099232/z/12/z]
- Biotechnology and Biological Sciences Research Council [1235154] Funding Source: researchfish
- European Research Council (ERC) [339778] Funding Source: European Research Council (ERC)
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We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability.
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