Journal
NATURE METHODS
Volume 13, Issue 8, Pages 657-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3895
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Funding
- US National Institute of Health single-cell analysis program [R01HD075605]
- Career Award at the Scientific Interface from the Burroughs Wellcome Fund
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Sequential barcoded fluorescent in situ hybridization (seqFISH) allows large numbers of molecular species to be accurately detected in single cells, but multiplexing is limited by the density of barcoded objects. We present correlation FISH (corrFISH), a method to resolve dense temporal barcodes in sequential hybridization experiments. Using corrFISH, we quantified highly expressed ribosomal protein genes in single cultured cells and mouse thymus sections, revealing cell-type-specific gene expression.
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