Journal
PLANTS-BASEL
Volume 12, Issue 14, Pages -Publisher
MDPI
DOI: 10.3390/plants12142590
Keywords
salt tolerance; marginal lands; photosynthesis; photosystem II; Brassica juncea; Brassica napus; relative gene expression
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Brassica juncea shows better tolerance to salt stress compared to Brassica napus, with higher photosynthetic potential and less ion accumulation. The reaction centers of PSII in B. juncea are more active and photoprotected, resulting in better efficiency and electron transport rates. Genetic differences, as reflected in the expression of key genes, contribute to the differential stress tolerance between these two Brassica species.
Brassica species show varying levels of resistance to salt stress. To understand the genetics underlying these differential stress tolerance patterns in Brassicas, we exposed two widely cultivated amphidiploid Brassica species having different genomes, Brassica juncea (AABB, n = 18) and Brassica napus (AACC, n = 19), to elevated levels of NaCl concentration (300 mM, half the salinity of seawater). B. juncea produced more biomass, an increased chlorophyll content, and fewer accumulated sodium (Na+) and chloride (Cl-) ions in its photosynthesizing tissues. Chlorophyll fluorescence assays revealed that the reaction centers of PSII of B. juncea were more photoprotected and hence more active than those of B. napus under NaCl stress, which, in turn, resulted in a better PSII quantum efficiency, better utilization of photochemical energy with significantly reduced energy loss, and higher electron transport rates, even under stressful conditions. The expression of key genes responsible for salt tolerance (NHX1 and AVP1, which are nuclear-encoded) and photosynthesis (psbA, psaA, petB, and rbcL, which are chloroplast-encoded) were monitored for their genetic differences underlying stress tolerance. Under NaCl stress, the expression of NHX1, D1, and Rubisco increased several folds in B. juncea plants compared to B. napus, highlighting differences in genetics between these two Brassicas. The higher photosynthetic potential under stress suggests that B. juncea is a promising candidate for genetic modifications and its cultivation on marginal lands.
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