Aronia melanocarpa Flavonol Extract-Antiradical and Immunomodulating Activities Analysis

The study of Aronia melanocarpa's (A. melanocarpa) biological activity is focused on obtaining the crude extract and separation of the flavonol compounds. The extraction and fractionation of A. melanocarpa fruits, followed by quantitative analysis, were accomplished using high-performance liquid chromatography and Darco G-60 filtering. This approach enabled the quantification of flavonoids within each fraction. The antioxidative, immunomodulating activities and cytotoxicity with respect to the lymphoblast cell line RPMI-1788 were studied. The flavonol extract of A. melanocarpa has been shown to have a high capacity to neutralize free DPPH and AAPH radicals in vitro. It also caused an accelerated 'respiratory burst' formation of neutrophils and an increase in the metabolic reserves of cells in rats exposed to cyclophosphamide. The reference solution (an equivalent quercetin-rutin blend) contributed to a decrease in lipid peroxidation, intensifying phagocytosis processes. The studied compounds demonstrated their low influence on the leukocyte blood profile in animals.

Automated summary

The study focused on the extraction and separation of flavonol compounds from Aronia melanocarpa fruits. High-performance liquid chromatography and Darco G-60 filtering were used for extraction and fractionation, followed by quantitative analysis to determine the flavonoid content in each fraction. The study revealed that the flavonol extract of A. melanocarpa has strong antioxidant activity, immunomodulating effects, and cytotoxicity against the lymphoblast cell line RPMI-1788. It also demonstrated the potential to enhance neutrophil activity and cell metabolism in rats exposed to cyclophosphamide, while showing minimal influence on the leukocyte blood profile in animals.