Solarplast(& REG;)-An Enzymatically Treated Spinach Extract

In the modern world we are constantly bombarded by environmental and natural stimuli that can result in oxidative stress. Antioxidant molecules and enzymes help the human body scavenge reactive oxygen species and prevent oxidative damage. Most organisms possess intrinsic antioxidant activity, but also benefit from the consumption of antioxidants from their diet. Leafy green vegetables such as spinach are a well-researched rich source of dietary antioxidant molecules. However, plant cell walls are difficult to digest for many individuals and the bio-accessibility of nutrients and antioxidants from these sources can be limited by the degree of digestion and assimilation. Through a specific enzymatic process, Solarplast((R)) contains organic spinach protoplasts without the cell wall, which may facilitate higher yield and efficacy of beneficial antioxidant molecules. In this study, analytical techniques coupled to in vitro bioassays were used to determine the potential antioxidant activity of Solarplast((R)) and determine its antioxidant enzymatic capabilities. Solarplast((R)) demonstrated superior antioxidant activity when compared to frozen spinach leaves in TOC, FRAP and TEAC antioxidant assays. Several antioxidant enzymes were also increased in Solarplast((R)), when compared to frozen spinach. As a functional readout, Solarplast((R)) attenuated hydrogen peroxide-, ethanol- and acetaminophen-induced increases in oxidative stress and cytotoxicity in both intestinal (HT-29) and liver (HepG2) cell lines. These findings suggest that Solarplast((R)) may represent a non-GMO, plant-based food supplement to help reduce oxidative stress in the human body.

Automated summary

In the modern world, our bodies are exposed to various environmental and natural stimuli that can lead to oxidative stress. Antioxidant molecules and enzymes play a crucial role in removing reactive oxygen species and protecting against oxidative damage. While most organisms have inherent antioxidant activity, dietary antioxidants from sources like leafy green vegetables, such as spinach, can also contribute to overall antioxidant levels. However, the bio-accessibility of nutrients and antioxidants from plant sources can be limited by the plant cell walls, making digestion and assimilation difficult. This study explores the potential of Solarplast((R)), a product derived from organic spinach protoplasts without the cell wall, in enhancing the antioxidant activity and efficacy of beneficial antioxidant molecules. The results show that Solarplast((R)) demonstrates superior antioxidant activity compared to frozen spinach leaves, as well as increased levels of antioxidant enzymes. Additionally, it attenuates oxidative stress and cytotoxicity induced by hydrogen peroxide, ethanol, and acetaminophen in intestinal and liver cell lines. These findings suggest that Solarplast((R)) could be a non-GMO, plant-based food supplement to help reduce oxidative stress in the human body.