Glandular and Non-Glandular Trichomes from Phlomis herba-venti subsp. pungens Leaves: Light, Confocal, and Scanning Electron Microscopy and Histochemistry of the Secretory Products

The purpose of this paper is to highlight the morphological peculiarities of glandular and non-glandular trichomes from leaves of Phlomis herba-venti subsp. Pungens using light, confocal, and scanning electron microscopy. Histochemistry techniques were used to analyze the localization of different chemical compounds in secretory trichomes. Two types of non-glandular trichomes were identified: unicellular and branched. They were found more frequently on the lower epidermis of leaves in different stages of ontogenetic development. Glandular trichomes were categorized as capitate (C1 and C2) with different stalk lengths and one-four secretory cells and dendroids (D) with one-four secretory cells. The histochemical analyses revealed distinct secretory products in terms of composition and distribution among the three types of glandular trichomes. The dendroid category of glandular trichomes is rarely found in plants and is not characteristic of the Lamiaceae species. They were described and characterized from a micromorphological and histochemical point of view for the first time in P. herba-venti.

Automated summary

The purpose of this study was to examine the morphological characteristics of glandular and non-glandular trichomes in leaves of Phlomis herba-venti subsp. Pungens using different microscopy techniques. Two types of non-glandular trichomes were identified: unicellular and branched, while glandular trichomes were categorized as capitate and dendroids. Histochemical analysis revealed distinct secretion products in each type of glandular trichomes. The dendroid trichomes were described and characterized for the first time in P. herba-venti.