Comparative Pollen Morphology of Selected Species of Blumea DC. and Cyathocline Cass. and Its Taxonomic Significance

The pollen morphology of 20 species from Blumea and Cyathocline Cass. was investigated using a light microscope (LM) and scanning electron microscopy (SEM) to explore their taxonomic significance. This study showed that pollen grains of these species were usually tricolporate, rarely tetracolporate (B. sinuata). Nine pollen types were distinguishable through the exine sculpture characters and the number of apertures. It was easily distinguished Cyathocline from species of Blumea s. str. by its much smaller size (15.04 mu m x 15.07 mu m) and sparse and longer spines (24 spines, spine length 4.23 mu m) with acute apex, which suggest that C. purpurea might not belong to the genus Blumea s. str. The palynological characteristics indicated that Section Macrophllae and Section Paniculatae of Blumea were not monophyletic groups. The pollen morphology differentiation of B. lacera clade is consistent with the interspecific relationship revealed by the molecular phylogenetic tree. However, the pollen morphology of the Blumea densiflora clade is inconsistent with the interspecific relationship based on molecular phylogenetic analysis. This palynology research can only partly support the previously published molecular phylogeny of Blumea s. str.

Automated summary

The pollen morphology of 20 species from Blumea and Cyathocline Cass. was investigated using a light microscope (LM) and scanning electron microscopy (SEM) to explore their taxonomic significance. Different types of pollen grains were distinguished based on their exine sculpture characters and the number of apertures. Cyathocline was easily distinguished from species of Blumea s. str. based on its smaller size and sparse and longer spines with acute apex. Palynological characteristics indicated that Section Macrophllae and Section Paniculatae of Blumea were not monophyletic groups.