Journal
PLANTS-BASEL
Volume 12, Issue 13, Pages -Publisher
MDPI
DOI: 10.3390/plants12132489
Keywords
Arabidopsis thaliana; pollen tube; lipid droplets; myosin; actin
Categories
Ask authors/readers for more resources
The growth of pollen tubes relies on actin filaments for reproduction in plants. Pharmacological experiments indicate that the trafficking of lipid droplets (LDs) in pollen tubes is related to F-actin. Additionally, Myo11C1 and Myo11C2 motors were found to be involved in LDs movement, sharing functional redundancy in Arabidopsis pollen tubes.
The growth of pollen tubes, which depends on actin filaments, is pivotal for plant reproduction. Pharmacological experiments showed that while oryzalin and brefeldin A treatments had no significant effect on the lipid droplets (LDs) trafficking, while 2,3-butanedione monoxime (BDM), latrunculin B, SMIFH2, and cytochalasin D treatments slowed down LDs trafficking, in such a manner that only residual wobbling was observed, suggesting that trafficking of LDs in pollen tube is related to F-actin. While the trafficking of LDs in the wild-type pollen tubes and in myo11-2, myo11b1-1, myo11c1-1, and myo11c2-1 single mutants and myo11a1-1/myo11a2-1 double mutant were normal, their trafficking slowed down in a myosin-XI double knockout (myo11c1-1/myo11c2-1) mutant. These observations suggest that Myo11C1 and Myo11C2 motors are involved in LDs movement in pollen tubes, and they share functional redundancy. Hence, LDs movement in Arabidopsis pollen tubes relies on the actomyosin system.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available