Journal
PLANTS-BASEL
Volume 12, Issue 15, Pages -Publisher
MDPI
DOI: 10.3390/plants12152763
Keywords
Phaseolus vulgaris L; molecular markers; DNA polymorphism; dendrogram; PCoA
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The study estimated the genetic diversity among 33 common bean genotypes using 10 RAPD and 10 SCoT markers. Both marker systems showed high levels of polymorphism, supported by high polymorphic information content (PIC) values. The efficiency of the markers was compared using the Diversity Detection Index (DDI) and Marker Index (MI), with SCoT markers showing higher values and greater efficiency in detecting molecular variability. The results confirm the separation of the bean genotypes and suggest the potential use of genetically distant material for breeding purposes.
Knowledge about the genetic diversity of the available common bean germplasm can help breeders properly direct the choice of genetic material in the breeding process. The aim of the present work was to estimate the usefulness of 10 RAPD and 10 SCoT markers in genetic diversity detection among 33 common bean genotypes. Both molecular marker systems were able to generate high levels of polymorphism in the genetic material, which was supported by the relatively high polymorphic information content (PIC) values observed for the used markers. The Diversity Detection Index (DDI) and Marker Index (MI) were used to compare the effectiveness of RAPD and SCoT markers. For both techniques, high values of MI and DDI were calculated, representing their effectivity. The SCoT markers showed higher values of the parameters used (MI = 7.474, DI = 2.265) than the RAPD markers (MI = 5.323, DDI = 1.612), indicating their higher efficiency in the detection of molecular variability. Three constructed dendrograms and PCoA plots were created using RAPD and SCoT, and both methods combined confirmed sufficient separation of the bean genotypes from each other. At the same time, a higher efficiency of SCoT markers compared to RAPD markers in the detection of the genetic diversity of beans was also proven. The results may be of future interest in the choice of genetically distant material for breeding purposes.
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