Journal
NATURE METHODS
Volume 13, Issue 12, Pages 989-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4046
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Funding
- Human Frontiers Science Program
- NSF Graduate Fellowship
- Siebel Scholar Award
- National Institute of Health [R01MH080047, 1DP1NS096787, 1U01NS090600, P50GM107615]
- Burroughs Wellcome Foundation Career Award for Medical Scientists
- Rita Allen Foundation Scholar Award
- Max Planck Florida Institute for Neuroscience
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We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay. When used together with EGFP-based biosensors, the new pair enables simultaneous imaging of the activities of two signaling molecules in single dendritic spines undergoing structural plasticity.
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