Journal
NATURE METHODS
Volume 13, Issue 12, Pages 985-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4034
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- Howard Hughes Medical Institute
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Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.
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