Fecal Carriage of Extended-Spectrum beta-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats

Dogs have been reported as potential carriers of antimicrobial-resistant bacteria, but the role of cats has been poorly studied. The aim of this study was to investigate the presence and the risk factors associated with the fecal carriage of extended-spectrum beta-lactamase and AmpC (ESBL/AmpC)-producing Escherichia coli (E. coli) in pet and stray cats. Fecal samples were collected between 2020 and 2022 from healthy and unhealthy cats and screened for ESBL/AmpC-producing E. coli using selective media. The presence of ESBL/AmpC-producing E. coli was confirmed by phenotypic and molecular methods. The evaluation of minimum inhibitory concentrations (MICs) was performed on positive isolates. Host and hospitalization data were analyzed to identify risk factors. A total of 97 cats' samples were collected, and ESBL/AmpC-producing E. coli were detected in 6/97 (6.2%), supported by the detection of bla(CTX-M) (100%), bla(TEM) (83.3%), and bla(SHV) (16.7%) genes and the overexpression of chromosomal ampC (1%). All E. coli isolates were categorized as multidrug-resistant. Unhealthy status and previous antibiotic therapy were significantly associated with ESBL/AmpC-producing E. coli fecal carriage. Our results suggest that cats may be carriers of ESBL/AmpC-producing E. coli, highlighting the need for antimicrobial stewardship in veterinary medicine and an antimicrobial-resistance surveillance program focusing on companion animals, including stray cats.

Automated summary

This study aimed to investigate the presence and risk factors associated with the fecal carriage of ESBL/AmpC-producing E. coli in cats. Fecal samples from pet and stray cats were screened and confirmed for the presence of ESBL/AmpC-producing E. coli. Multidrug-resistant isolates were detected, and unhealthy cats and previous antibiotic therapy were significantly associated with fecal carriage of ESBL/AmpC-producing E. coli. These findings highlight the importance of antimicrobial stewardship and surveillance programs in companion animals, including stray cats.